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Reproducibility of human epidermal growth factor receptor 2 analysis in primary breast cancer - A national survey performed at pathology departments in Sweden
Lund University Hospital.
Malmö University Hospital.
Lund University Hospital.
Karolinska University Hospital.
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2009 (Engelska)Ingår i: ACTA ONCOLOGICA, ISSN 0284-186X, Vol. 48, nr 6, s. 860-866Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background. HER2 is a treatment predictive factor for the effect of trastuzumab and associated with poor prognosis in breast cancer. The analysis of HER2 must be performed with good quality, with regard to both the immunohistochemical (IHC) and in situ hybridization (ISH) analysis. Material and methods. A tissue microarray (TMA) including 11 breast cancer samples was sent twice (once in 2005 and again in 2006) to 24 pathology departments in Sweden. A questionnaire was also sent to the departments in 2006. Results. With IHC, all departments reported the same results (0/1+ vs. 2+ vs. 3+) for three (2005) and six samples (2006). The mean kappa-value increased from 0.67 to 0.77, indicating a good reproducibility at both occasions. With fluorescence-ISH (FISH), the 11 departments using this technique reported the same results (amplified vs. normal) for nine (2005) and ten samples (2006). The mean kappa-value showed very good reproducibility both 2005 and 2006 (0.92 and 0.96, respectively). Based on the answers from the participating departments, the questionnaire revealed that 31% of primary breast cancer diagnosed in 2006 (n = 5 043) were 2+/3+. FISH analysis of 2+ confirmed 12% of the samples to be amplified. The corresponding figure for 3+ was 90%. In total, 14.3% of the samples were HER2 positive (2+ and amplified, or 3+). Discussion. The results obtained in this study indicate that the reproducibility for HER2 analysis is good (IHC) and very good (FISH) between the pathology departments in Sweden using TMA-based tumor samples. In 2006, 14.3% of invasive breast cancers were HER2 positive.

Ort, förlag, år, upplaga, sidor
2009. Vol. 48, nr 6, s. 860-866
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-20418OAI: oai:DiVA.org:liu-20418DiVA, id: diva2:234528
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DOI does not work: 10.1080/02841860902862511

Tillgänglig från: 2009-09-08 Skapad: 2009-09-07 Senast uppdaterad: 2018-03-09

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Olsson, Hans

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Olsson, Hans
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Molekylär och immunologisk patologiHälsouniversitetetKlinisk patologi och klinisk genetik
Medicin och hälsovetenskap

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