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Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
2008 (Engelska)Ingår i: BIOINTERPHASES, ISSN 1559-4106 , Vol. 3, nr 3, s. 75-82Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly (dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain.

Ort, förlag, år, upplaga, sidor
2008. Vol. 3, nr 3, s. 75-82
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-17907DOI: 10.1116/1.2988771OAI: oai:DiVA.org:liu-17907DiVA, id: diva2:212995
Tillgänglig från: 2009-04-26 Skapad: 2009-04-24 Senast uppdaterad: 2010-04-20
Ingår i avhandling
1. Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
Öppna denna publikation i ny flik eller fönster >>Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2010. s. 60
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1308
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:liu:diva-54902 (URN)978-91-7393-408-4 (ISBN)
Disputation
2010-05-14, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 14:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2010-04-20 Skapad: 2010-04-20 Senast uppdaterad: 2020-02-19Bibliografiskt granskad

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Wigenius, JensFransson, Sophiavon Post, FredrikInganäs , Olle

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Wigenius, JensFransson, Sophiavon Post, FredrikInganäs , Olle
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Biomolekylär och Organisk ElektronikTekniska högskolanInstitutionen för fysik, kemi och biologi
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