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Methods for integrating fluorimetry in the study of hearing organ structure and function
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
Huddinge Hospital, Sweden.
1997 (Engelska)Ingår i: Hearing Research, ISSN 0378-5955, E-ISSN 1878-5891, Vol. 106, nr 1-2, s. 29-38Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The measurement of function in the intact organ of Corti has up to now been achieved by three methods: electrophysiology, mechanical measurement and biochemical analysis. The two former methods have supplied information at the level of single identified cells. We have used a fourth method, optical fluorimetry, to measure hair cell function at the cellular level in the intact organ of Corti. Here we describe the methods involved in fluorescence labelling and video-enhanced microscopy in combination with electrophysiological recording of cochlear microphonic (CM) and summating potentials (SP). The guinea pig temporal bone containing an intact ear drum, ossicular chain and cochlea can be maintained in the isolated state by perfusion of the scala tympani with oxygenated tissue culture medium. Substances added to the perfusate readily diffuse through the basilar membrane into the organ of Corti. In this way cells in the organ can be stained by a number of fluorescent probes which label different structures and functions. Here we have used two dyes which label mitochondria and fluoresce with an intensity proportional to metabolic activity. By simultaneous measurement of CM and SP the functional state of the organ can be monitored.

Ort, förlag, år, upplaga, sidor
Elsevier, 1997. Vol. 106, nr 1-2, s. 29-38
Nyckelord [en]
Auditory system; Hearing; Organ of Corti; Hair cell; Fluorescence microscopy; Cochlear potential
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Neurovetenskaper
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URN: urn:nbn:se:liu:diva-101082PubMedID: 9112105OAI: oai:DiVA.org:liu-101082DiVA, id: diva2:665127
Tillgänglig från: 2013-11-19 Skapad: 2013-11-19 Senast uppdaterad: 2018-01-11Bibliografiskt granskad

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