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A red- emissive mitochondrial probe for imaging of the viscosity in living cells
Wannan Med Coll, Peoples R China.
Wannan Med Coll, Peoples R China.
Wannan Med Coll, Peoples R China.
Wannan Med Coll, Peoples R China.
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2019 (Engelska)Ingår i: New Journal of Chemistry, ISSN 1144-0546, E-ISSN 1369-9261, Vol. 43, nr 22, s. 8811-8815Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A novel water- soluble fluorescent probe L based on indole salts has been designed, synthesized and fully characterized. The systematic investigations demonstrated that probe L shows red emission and the fluorescence intensity is linear with the viscosity of the medium. Probe L is able to selectively accumulate in mitochondria within 1 min without any additional reagents for membrane permeabilization. It has been used to distinguish the viscosity differences between mitochondria in normal and nystatin- treated HeLa cells. In addition, due to the good photostability, probe L can be used to monitor the dynamics of mitochondria. These results support that probe L might provide a promising approach for the fluorescence detection of mitochondrial viscosity in living biological systems.

Ort, förlag, år, upplaga, sidor
ROYAL SOC CHEMISTRY , 2019. Vol. 43, nr 22, s. 8811-8815
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Organisk kemi
Identifikatorer
URN: urn:nbn:se:liu:diva-158866DOI: 10.1039/c9nj01826cISI: 000470919700049OAI: oai:DiVA.org:liu-158866DiVA, id: diva2:1337593
Anmärkning

Funding Agencies|Anhui Provincial Natural Science Foundation [1908085QB50]; Doctoral Startup Foundation of Wannan Medical College [wYRCQD201715]; Young Foundation of Wannan Medical College [WK201807]; Natural Science Fund of Education Department of Anhui province [KJ2018A0257]; National Natural Science Foundation of China [21602157]; Swedish Government strategic faculty grant in material science (SFO, MATLIU) in Advanced Functional Materials (AFM) (VR) [5.1-2015-5959]; Scientific Research Foundation for Academic and Technical Leader Reserve Candidate of Anhui Province [2016H086]

Tillgänglig från: 2019-07-16 Skapad: 2019-07-16 Senast uppdaterad: 2019-07-16

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Uvdal, KajsaHu, Zhang-Jun
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