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The use of FTA cards to acquire DNA profiles from postmortem cases
Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för läkemedelsforskning. Linköpings universitet, Medicinska fakulteten. Natl Board Forens Med, Dept Forens Genet and Forens Toxicol, Artillerigatan 12, S-58758 Linkoping, Sweden.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för hematopoes och utvecklingsbiologi. Linköpings universitet, Medicinska fakulteten. Natl Board Forens Med, Dept Forens Genet and Forens Toxicol, Artillerigatan 12, S-58758 Linkoping, Sweden.
Natl Board Forens Med, Sweden.
Natl Board Forens Med, Dept Forens Genet and Forens Toxicol, Artillerigatan 12, S-58758 Linkoping, Sweden.
2019 (Engelska)Ingår i: International journal of legal medicine (Print), ISSN 0937-9827, E-ISSN 1437-1596, Vol. 133, nr 6, s. 1651-1657Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Filter papers have been used for many years in different applications of molecular biology and have been proven to be a stable way to store DNA waiting to be analyzed. Sampling of DNA on FTA (Flinders Technology Associates) cards is convenient and cost effective compared to alternative approaches involving DNA extractions and storage of DNA extracts. FTA cards are analyzed at many forensic laboratories, and the way to perform direct genetic profiling on buccal swab cards has developed into an almost industrial process. The possibility to include postmortem (PM) samples into an FTA-based workflow would facilitate and speed up the genetic identification process compared to conventional methods, both on a regular basis and in a mass casualty event. In this study, we investigated if FTA cards may be used to carry tissue DNA from deceased and present a high-quality DNA profile from the individual in order to be useful for the identification process. The study also aimed to investigate if a specific body tissue would be preferable, and if decomposed tissue is suitable at all to put on an FTA card in order to obtain a DNA profile. We have compared the quality of the DNA profiles acquired from postmortem tissue on FTA cards, with the results acquired with conventional methods from reference bone/muscle samples from the same individual. Several types of tissues have been tested from different identification cases and scenarios. We concluded that tissue cells from inner organs are suitable to put on FTA cards, and that the obtained DNA profiles have the potential to serve as PM data for identification purposes. In cases including compromised samples, however, it is recommended to keep the tissue sample as a backup if further DNA has to be extracted.

Ort, förlag, år, upplaga, sidor
SPRINGER , 2019. Vol. 133, nr 6, s. 1651-1657
Nyckelord [en]
DNA; FTA; Postmortem; Disaster victim identification; Mass disaster; Human identification
Nationell ämneskategori
Genetik
Identifikatorer
URN: urn:nbn:se:liu:diva-161837DOI: 10.1007/s00414-019-02015-2ISI: 000492038100003PubMedID: 30747256OAI: oai:DiVA.org:liu-161837DiVA, id: diva2:1370906
Anmärkning

Funding Agencies|National Board of Forensic Medicine

Tillgänglig från: 2019-11-18 Skapad: 2019-11-18 Senast uppdaterad: 2019-11-18

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Green, HenrikTillmar, Andreas
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Avdelningen för läkemedelsforskningMedicinska fakultetenAvdelningen för hematopoes och utvecklingsbiologi
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International journal of legal medicine (Print)
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