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Protein profiling of low-density lipoprotein from obese subjects
Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Medicincentrum, Arbets- och miljömedicin. Östergötlands Läns Landsting, Medicincentrum.
n/a.
Chalmers.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Yrkes- och miljömedicin. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Medicincentrum, Arbets- och miljömedicin.
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2009 (Engelska)Ingår i: PROTEOMICS CLINICAL APPLICATIONS, ISSN 1862-8346, Vol. 3, nr 6, s. 663-671Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Although obesity and high levels of low-density lipoprotein (LDL) are well-known risk factors for cardiovascular disease, the precise role(s) of different LDL constituents in obesity has not been explored. In the present study, we compared the LDL proteome of healthy control adults (body mass index less than 25) and obese subjects (body mass index greater than 30). LDL was isolated by density-gradient ultracentrifugation and proteins were separated with 2-D PAGE, quantified, and identified by peptide mass fingerprinting using MALDI-TOF MS. A new LDL-associated protein was identified as transthyretin and found to be significantly more abundant in LDL from the obese subjects. In addition, LDL from the obese subjects contained relatively more alpha(1)-antitrypsin, apo J, apo C-II, than LDL from controls, and also more of an acidic isoform (pI/Mr; 5.2/23 100) of apo A-I. On the other hand, the relative amounts of apo A-IV and the major isoform of apo A-I (pI/Mr; 5.3/23 100) were significantly less in LDL from the obese subjects. Apo E was less and non-sialylated apo C-III more abundant in LDL from obese men than control men, while there were no such differences between LDL from obese and control women. These findings illustrate that obesity is not only associated with increased LDL-cholesterol levels but also with alterations in the LDL protein composition. The presence of transthyretin in LDL from obese subjects may reflect over-nutrition and affect the lipid metabolism in obesity.

Ort, förlag, år, upplaga, sidor
2009. Vol. 3, nr 6, s. 663-671
Nyckelord [en]
2-DE; Low-density lipoprotein; Obesity; Proteomics; Transthyretin
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-20158DOI: 10.1002/prca.200800138OAI: oai:DiVA.org:liu-20158DiVA, id: diva2:233669
Tillgänglig från: 2009-09-01 Skapad: 2009-08-31 Senast uppdaterad: 2010-01-09

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Karlsson, HelenTagesson, ChristerLindahl, Mats

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Institutionen för klinisk och experimentell medicinHälsouniversitetetArbets- och miljömedicinMedicincentrumYrkes- och miljömedicin
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