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Efficient imaging of amyloid deposits in Drosophila models of human amyloidoses
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biokemi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0002-5582-140X
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Utvecklingsbiologi. Linköpings universitet, Hälsouniversitetet.ORCID-id: 0000-0001-5095-541X
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biokemi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-5827-3587
2010 (Engelska)Ingår i: Nature Protocols, ISSN 1754-2189, E-ISSN 1750-2799, Vol. 5, nr 5, s. 935-944Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Drosophila melanogaster is emerging as an important model system for neurodegenerative disease research. In this protocol, we describe an efficient method for imaging amyloid deposits in the Drosophila brain, by the use of a luminescent-conjugated oligothiophene (lco), p-Ftaa polymer probe. We also demonstrate the feasibility of co-staining with antibodies and compare the lco staining with standard amyloid-specific probes. the lco protocol enables high-resolution imaging of several different protein aggregates, such as aβ1-42, aβ1-42e22G, transthyretin V30M and human tau, in the Drosophila brain. aβ and tau aggregates could also be distinguished from each other because of distinct lco emission spectra. Furthermore, this protocol enables threedimensional brain mapping of amyloid distribution in whole-mount Drosophila brains. the use of p-Ftaa combined with other probes, antibodies and/or dyes will aid the rapid characterization of various amyloid deposits in the rapidly growing number of Drosophila models of neurodegenerative diseases.

Ort, förlag, år, upplaga, sidor
Macmillan Publishers Ltd., 2010. Vol. 5, nr 5, s. 935-944
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-55024DOI: 10.1038/nprot.2010.41ISI: 000277174100012OAI: oai:DiVA.org:liu-55024DiVA, id: diva2:314356
Tillgänglig från: 2010-04-27 Skapad: 2010-04-27 Senast uppdaterad: 2018-04-25Bibliografiskt granskad
Ingår i avhandling
1. Modeling Amyloid Disease in Drosophila melanogaster
Öppna denna publikation i ny flik eller fönster >>Modeling Amyloid Disease in Drosophila melanogaster
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Amyloid diseases are caused by protein misfolding and aggregation. To date there are 27 known proteins causing amyloid disorders involving brain and peripheral protein deposition. The proteins involved in this mechanism do not share sequence homology, but the amyloid fibrils share biophysical properties and possibly a common pathogenic mechanism. Amyloid deposits are known to be involved in a broad range of neurodegenerative diseases, such as Alzheimer’s disease and Creutzfeldt-Jakob disease, as well as in non-neuropathic diseases, such as senile systemic amyloidosis and type II diabetes.

During the last decade the fruit fly, Drosophila melanogaster (Drosophila), have increasingly been used as a model for neurodegenerative disease, such as Alzheimer’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and familial amyloidotic polyneuropathy. The advantages of using the Drosophila model are the well-defined genetic characteristics, the quantity, short life span, simplicity in genetic manipulation and the powerful binary UAS-Gal4 transgenic system. The UAS-Gal4 system allows for rapid generation of individual strains in which expression of a specific gene of interest can be directed to different tissues or cell types. The system allows the target gene to be activated in different cell- and tissue-types by altering the activator-expressing lines.

This thesis has been focused on modeling amyloid diseases in Drosophila. This has been performed by:

  • Creating new model systems of senile systemic amyloidosis and familial amyloidotic polyneuropathy in Drosophila
  • Developing a new staining protocol for detection of amyloid in Drosophila
  • Initiate a compound screen of Alzheimer’s disease modeled in Drosophila
Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2010. s. 88
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1320
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:liu:diva-55025 (URN)978-91-7393-379-7 (ISBN)
Disputation
2010-05-21, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 09:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2010-04-27 Skapad: 2010-04-27 Senast uppdaterad: 2020-02-19Bibliografiskt granskad

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Berg, InaNilsson, PeterThor, StefanHammarström, Per

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