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A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors
Cranfield University, Cranfield Health, Cranfield, Bedfordshire, UK.
EPFL, IMT-SAMLAB, Switzerland.
EPFL, IMT-SAMLAB, Switzerland.
EPFL, IMT-SAMLAB, Switzerland.
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2010 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, nr 4, s. 1582-1587Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The present work investigates the utilisation of the widely used SU-8 photoresist as an immobilisation matrix for glucose oxidase (GOx) for the development of glucose micro-biosensors. The strong advantage of the proposed approach is the simultaneous enzyme entrapment during the microfabrication process within a single step, which is of high importance for the simplification of the BioMEMS procedures. Successful encapsulation of the enzyme GOx in "customised" SU-8 microfabricated structures was achieved through optimisation of the one-step microfabrication process. Although the process involved contact with organic solvents, UV-light exposure, heating for pre- and post-bake and enzyme entrapment in a hard and rigid epoxy resin matrix, the enzyme retained its activity after encapsulation in SU-8. Measurements of the immobilised enzymes activity inside the SU-8 matrix were carried out using amperometric detection of hydrogen peroxide in a 3-electrode setup. Films without enzyme showed negligible variation in current upon the addition of glucose, as opposed to films with encapsulated enzyme which showed a very clear increase in current. Experiments using films of increased thickness or enzyme concentration, showed a higher response, thus proving that the enzyme remained active not only on the films surface, but also inside the matrix as well. The proposed enzyme immobilisation in SU-8 films opens up new possibilities for combining BioMEMS with biosensors and organic electronics.

Ort, förlag, år, upplaga, sidor
Elsevier , 2010. Vol. 26, nr 4, s. 1582-1587
Nyckelord [en]
SU-8 films; Biosensor; Microfabrication; Immobilisation; Glucose oxidase entrapment; BioMEMS
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Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:liu:diva-70596DOI: 10.1016/j.bios.2010.07.117ISI: 000286403400067OAI: oai:DiVA.org:liu-70596DiVA, id: diva2:440908
Tillgänglig från: 2011-09-14 Skapad: 2011-09-14 Senast uppdaterad: 2017-12-08

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