liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Fibrin Sealants from Fresh or Fresh/Frozen Plasma as Scaffolds for In Vitro Articular Cartilage Regeneration
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada..
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada..ORCID-id: 0000-0003-1222-6720
Orthopaedic Biomechanics Laboratory, CHEO Research Institute, Ottawa, Ontario, Canada.
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada..
Visa övriga samt affilieringar
2009 (Engelska)Ingår i: TISSUE ENGINEERING PART A, ISSN 1937-3341, Vol. 15, nr 8, s. 2285-2297Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Our objective was to evaluate human CryoSeal (R) fibrin glue derived from single units of plasma as scaffolds for articular cartilage tissue engineering. Human articular chondrocytes were encapsulated into genipin cross-linked fibrin glue derived from individual units of fresh or frozen plasma using the CryoSeal (R) fibrin sealant (FS) system. The constructs were cultured for up to 7 weeks in vitro under low (5%) or normal (21%) oxygen. Chondrocyte viability was greater than90% within the fibrin gels. Hypoxia induced significant increases in collagen II and Sox9 gene expression and a significant decrease in collagen I. A significant increase in collagen II was detected in fresh plasma-derived cultures, while only collagen I was significantly increased in frozen plasma cultures. Significant increases in total glycosaminoglycan and collagen were detected in the extracellular matrix secreted by the encapsulated chondrocytes. A significant increase in compression modulus was only observed for fresh plasma-derived gels, which is likely explained by a greater amount of collagen type I detected after 7 weeks in frozen compared to fresh plasma gels. Our results indicate that CryoSeal (R) fibrin glue derived from fresh plasma is suitable as a tissue engineering scaffold for human articular chondrocytes, and therefore should be evaluated for autologous articular cartilage regeneration.

Ort, förlag, år, upplaga, sidor
Mary Ann Leibert Inc , 2009. Vol. 15, nr 8, s. 2285-2297
Nationell ämneskategori
Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:liu:diva-70673DOI: 10.1089/ten.tea.2008.0228ISI: 000268703400038OAI: oai:DiVA.org:liu-70673DiVA, id: diva2:441034
Tillgänglig från: 2011-09-14 Skapad: 2011-09-14 Senast uppdaterad: 2013-12-17

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltext

Personposter BETA

Griffith, May

Sök vidare i DiVA

Av författaren/redaktören
Griffith, May
Teknik och teknologier

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 56 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf