liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions
Ryhov Hospital, Sweden .
Ryhov Hospital, Sweden .
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Dermatologi och venerologi. Linköpings universitet, Hälsouniversitetet.
Helmholtz Centre Infect Research, Germany .
Visa övriga samt affilieringar
2012 (Engelska)Ingår i: Journal of Inflammation, ISSN 1476-9255, E-ISSN 1476-9255, Vol. 9, nr 43Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Gold salts has previously been used in the treatment of rheumatoid arthritis but have been replaced by biologicals such as TNF-alpha inhibitors. The mechanisms behind the anti-inflammatory effect of metallic gold ions are still unknown, however, recent data showed that charged gold atoms are released from pure metallic gold implants by macrophages via a dissolucytosis membrane, and that gold ions are taken up by local macrophages, mast cells and to some extent fibroblasts. These findings open the question of possible immunomodulatory effects of metallic gold and motivate efforts on a deeper understanding of the effect of metallic gold on key inflammatory cells as macrophages. less thanbrgreater than less thanbrgreater thanMethods: Human macrophage cells (cell line THP-1) were grown on gold foils and intracellular uptake was analysed by autometallography. The impact of phagocytised gold ions on viability of THP-1 cells was investigated by trypan blue staining and TUNEL assay. The global gene expression profile of THP-1 cells after incorporation of gold ions was studied using microarray analysis comprising approximately 20,000 genes. The gene expression data was confirmed by measurement of secreted proteins. less thanbrgreater than less thanbrgreater thanResults: Autometallography showed intracellular uptake of gold ions into THP-1 cells. No significant effect on viability of THP-1 cells was demonstrated. Our data revealed a unique gene expression signature of dissolucytotic THP-1 cells that had taken up gold ions. A large number of regulated genes were functionally related to immunomodulation. Gold ion uptake induced downregulation of genes involved in rheumatoid arthritis such as hepatocyte growth factor, tenascin-C, inhibitor of DNA binding 1 and 3 and matrix metalloproteinase 13. less thanbrgreater than less thanbrgreater thanConclusion: The data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

Ort, förlag, år, upplaga, sidor
BioMed Central , 2012. Vol. 9, nr 43
Nyckelord [en]
Gold, Macrophages, Inflammation, Rheumatoid arthritis
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-87469DOI: 10.1186/1476-9255-9-43ISI: 000312597100001OAI: oai:DiVA.org:liu-87469DiVA, id: diva2:589464
Tillgänglig från: 2013-01-18 Skapad: 2013-01-18 Senast uppdaterad: 2017-12-06

Open Access i DiVA

fulltext(1614 kB)205 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1614 kBChecksumma SHA-512
cda6de9143a535003cfe4c9d02213cf2cb375ba0f9b039aea964550e4fe2faab73ceb93eec4d81f58e77a7946178469296316a255e5cf98f3fe91dd082659427
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Personposter BETA

Sjögren, FlorenceAnderson, Chris

Sök vidare i DiVA

Av författaren/redaktören
Sjögren, FlorenceAnderson, Chris
Av organisationen
Dermatologi och venerologiHälsouniversitetetHudkliniken i Östergötland
I samma tidskrift
Journal of Inflammation
Medicin och hälsovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 205 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 142 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf