liu.seSök publikationer i DiVA
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Persistent organic pollutants distribution in lipoprotein fractions in relation to cardiovascular disease and cancer.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för inflammationsmedicin. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för inflammationsmedicin. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärt- och Medicincentrum, Arbets- och miljömedicin.
Örebro University, Sweden.
Örebro University, Sweden.
Visa övriga samt affilieringar
2014 (Engelska)Ingår i: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 65, s. 93-9Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Persistent organic pollutants (POPs) are lipophilic environmental toxins that have been associated with cardiovascular disease (CVD) and cancer. The aim of this study was to investigate the concentrations of POPs in human high and low/very low-density lipoproteins (HDL and LDL/VLDL) and the possible association with CVD and cancer occurrence in individuals living in a contaminated area. Lipoproteins from 28 individuals (7 healthy controls, 8 subjects with cancer, 13 subjects with CVD) were isolated and the fraction-specific concentration of 20 different POPs was analyzed by high resolution gas chromatography/high resolution mass spectrometry. The activity of Paraoxonase 1 (PON1), an anti-oxidant in HDL, was determined in plasma of these 28 subjects and additional 50 subjects from the same area excluding diseases other than cancer or CVD. Fourteen polychlorinated biphenyls (PCBs) and three organochlorine pesticides were detected, and especially highly chlorinated PCBs were enriched in lipoproteins. Significantly higher concentrations of POPs were found among individuals with CVD or cancer compared to controls. Principal component analyses showed that POP concentrations in HDL were more associated with CVD, while POP concentrations in LDL/VLDL were more associated with cancer. PON1 activity was negatively correlated to sumPCB and a co-variation between decreased arylesterase-activity, increased PCB concentrations and CVD was found. This study shows that POPs are present in lipoproteins and were more abundant in individuals with CVD or cancer compared to healthy controls. The results also indicate that PCB exposure is accompanied by reduced PON1 activity that could impair the HDL function to protect against oxidation.

Ort, förlag, år, upplaga, sidor
Elsevier, 2014. Vol. 65, s. 93-9
Nationell ämneskategori
Klinisk medicin Annan medicinsk grundvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-104755DOI: 10.1016/j.envint.2013.12.017ISI: 000334728500010PubMedID: 24472825OAI: oai:DiVA.org:liu-104755DiVA, id: diva2:698789
Tillgänglig från: 2014-02-25 Skapad: 2014-02-25 Senast uppdaterad: 2018-01-11
Ingår i avhandling
1. Lipoproteomics: Environmental and Genetic Factors Affecting High-Density Lipoprotein (HDL)
Öppna denna publikation i ny flik eller fönster >>Lipoproteomics: Environmental and Genetic Factors Affecting High-Density Lipoprotein (HDL)
2016 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Lipoprotein particles act as lipid transporters in the blood stream, and measuring cholesterol content in specific subclasses of lipoprotein particles has long been, and still is, a frequently used tool to estimate the risk of cardiovascular disease (CVD). High-density lipoprotein (HDL) is a subclass of lipoproteins often regarded as providing protection against CVD via several functions including reverse cholesterol transport and anti-inflammatory capacities. However, the precise relationship between HDL cholesterol levels and health outcome is still unclear. Lately, new approaches to study HDL composition and function have therefore become more important.

HDL function is to a large extent dependent on its proteome, containing more than 100 proteins. Investigating the proteome in individuals with altered gene expression for HDL-associated proteins or with known exposure to environmental contaminants may reveal new insights into how HDL metabolism is affected by various factors. This is of interest in order to better understand the role of HDL in CVD.

Papers I and II focus on two different mutations in a structural HDL protein, apolipoprotein A-I (L202P and K131del), and one mutation in the scavenger receptor class B-1 (P297S), which is involved in selective lipid uptake of cholesterol mainly into hepatocytes and adrenal cells. The HDL proteome was analyzed using two-dimensional gel electrophoresis and mass spectrometry. The L202P mutation was identified in HDL of the heterozygote carriers together with a significant decrease of apolipoprotein E and increased zinc-alpha-2-glycoprotein. By contrast, the second apolipoprotein AI mutation (K131del) was associated with significantly elevated alpha-1-antitrypsin and transthyretin levels. Protein analyses of the scavenger receptor class B1 P297S heterozygotes showed a significant increase in HDL apoL-1 along with increased free apoE. The carriers showed no difference in antioxidative capability but a significant increase in apoA-I methionine oxidation.

Papers III and IV focus on persistent organic pollutants that may influence HDL composition and function. These compounds accumulate in humans, and exposure has been linked to an increased risk of CVD. To provide a better understanding of the HDL system in relation to pollutants, a population living in a contaminated area was studied. Persistent organic pollutants in isolated HDL were quantified using high-resolution gas chromatography mass spectrometry and significantly increased levels were found in individuals with CVD as compared to healthy controls. Furthermore, there was a significant negative association between the pollutants and paraoxonase-1 anti-oxidant activity. Studying the proteome with nano-liquid chromatography tandem mass spectrometry led to the identification of 118 proteins in HDL, of which ten were significantly associated with the persistent organic pollutants.

In summary, the present studies demonstrate protein pattern alterations in HDL associated with inherited genetic variants or pollutant exposure. The studies also provide a set of methods that are useful tools to further comprehend the complexity of lipoprotein metabolism and function. The results are important in order to improve our understanding of HDL in CVD and to explain an increased risk of CVD associated with exposure to organic pollutants.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2016. s. 56
Serie
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1531
Nationell ämneskategori
Biokemi och molekylärbiologi Läkemedelskemi Farmakologi och toxikologi Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) Medicinsk genetik
Identifikatorer
urn:nbn:se:liu:diva-131643 (URN)10.3384/diss.diva-131643 (DOI)9789176857090 (ISBN)
Disputation
2016-11-04, Birgittasalen, Hälsans hus, Campus US, Linköping, 09:00 (Svenska)
Opponent
Handledare
Tillgänglig från: 2016-09-30 Skapad: 2016-09-30 Senast uppdaterad: 2019-10-29Bibliografiskt granskad

Open Access i DiVA

fulltext(773 kB)935 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 773 kBChecksumma SHA-512
4d53b0a4270b9a6234bb92d86131fa83b60f1d10d2f4765f09384218dc612523f3ebf6274686e37ea5e2cdc7c10edc81887540e57477234bd9dc8dd769633ae4
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Personposter BETA

Ljunggren, Stefan AHelmfrid, IngelaWingren, GunLindahl, MatsKarlsson, Helen

Sök vidare i DiVA

Av författaren/redaktören
Ljunggren, Stefan AHelmfrid, IngelaWingren, GunLindahl, MatsKarlsson, Helen
Av organisationen
Avdelningen för inflammationsmedicinHälsouniversitetetArbets- och miljömedicin
I samma tidskrift
Environment International
Klinisk medicinAnnan medicinsk grundvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 935 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 401 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf