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Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska högskolan. (Integrative Regenerative Medicine (IGEN) Centre)
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet. Karolinska Institutet, Stockholm, Sweden. (Integrative Regenerative Medicine (IGEN) Centre)
Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan. (Integrative Regenerative Medicine (IGEN) Centre)
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska högskolan. (Integrative Regenerative Medicine (IGEN) Centre)
Visa övriga samt affilieringar
2014 (Engelska)Ingår i: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 102, nr 7, s. 1553-1561Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The myocardium is unable to regenerate itself after infarct, resulting in scarring and thinning of the heart wall. Our objective was to develop a patch to buttress and bypass the scarred area, while allowing regeneration by incorporated cardiac stem/progenitor cells (CPCs). Polycaprolactone (PCL) was fabricated as both sheets by solvent casting, and fibrous meshes by electrospinning, as potential patches, to determine the role of topology in proliferation and phenotypic changes to the CPCs. Thiophene-conjugated carbon nanotubes (T-CNTs) were incorporated to enhance the mechanical strength. We showed that freshly isolated CPCs from murine hearts neither attached nor spread on the PCL sheets, both with and without T-CNT. As electrospun meshes, however, both PCL and PCL/T-CNT supported CPC adhesion, proliferation, and differentiation. The incorporation of T-CNT into PCL resulted in a significant increase in mechanical strength but no morphological changes to the meshes. In turn, proliferation, but not differentiation, of CPCs into cardiomyocytes was enhanced in T-CNT containing meshes. We have shown that changing the topology of PCL, a known hydrophobic material, dramatically altered its properties, in this case, allowing CPCs to survive and differentiate. With further development, PCL/T-CNT meshes or similar patches may become a viable strategy to aid restoration of the postmyocardial infarction myocardium.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2014. Vol. 102, nr 7, s. 1553-1561
Nyckelord [en]
topology, carbon nanotubes, polycaprolactone, cardiac progenitor cells, electrospun meshes
Nationell ämneskategori
Klinisk medicin Medicinska och farmaceutiska grundvetenskaper Fysik
Identifikatorer
URN: urn:nbn:se:liu:diva-111488DOI: 10.1002/jbm.b.33136ISI: 000342963000020PubMedID: 24664884OAI: oai:DiVA.org:liu-111488DiVA, id: diva2:756733
Tillgänglig från: 2014-10-19 Skapad: 2014-10-19 Senast uppdaterad: 2018-01-11Bibliografiskt granskad
Ingår i avhandling
1. Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration
Öppna denna publikation i ny flik eller fönster >>Multifunctional Biomimetic Scaffolds Tailored for Cardiac Regeneration
2015 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Nature has had millions of years to perfect the structural components of the human body, but has also produced the dysfunctions that result in the cancers and diseases, which ruin that perfection. Congenital heart defects, and myocardial infarction lead to scarring that remodels heart muscle, decreasing the contractility of the heart, with profound consequences for the host. Regenerative medicine is the study of strategies to return diseased body parts to their evolutionarily optimum structure.

Nature has had millions of years to perfect the structural components of the human body, but has also produced the dysfunctions that result in the cancers and diseases, which ruin that perfection. Congenital heart defects, and myocardial infarction lead to scarring that remodels heart muscle, decreasing the contractility of the heart, with profound consequences for the host. Regenerative medicine is the study of strategies to return diseased body parts to their evolutionarily optimum structure. Cells alone cannot develop into functional tissue, as they require mechanical support and chemical signals from the extracellular matrix in order to play the correct role in the body. In order to imitate the process of tissue formation optimized by nature, scaffolds are developed as the architectural support for tissue regeneration. To mimic the elasticity and strength seen in the heart muscle is one of the major scientific conundrums of our time. The development of new multifunctional materials for scaffolds is an accepted solution for repairing failing heart muscle. In this thesis I accept the notion that endogenous cardiac cells can play a major role in addressing this problem, if we can attract them to the site of defect or injury and make them proliferate. I then proceed to show how improving on a commonly used synthetic polymer was used to develop two new biomaterials.

Polycaprolactone (PCL) fibers and sheets were studied for their ability to adsorb proteins based on their surface energies. We found that although the wettability of the PCL might be similar to positive controls for cell attachment, the large differences in surface energies may account for the increased serum protein adsorption and limit cell adhesion. The effect of fiber morphology was then investigated with respect to proliferation of mesenchymal stem cells and cardiac progenitor cells. PCL was also mechanically enhanced with thiophene conjugated single walled carbon nanotubes (T-CNT); where small concentrations of the T-CNT allowed for a 2.5 fold increase in the percentage of elongation, while retaining the proliferation profile of the cardiac progenitor cells. Although PCL is a well-known implant material, the ability to attract and adhere cardiac cells was limited. Therefore we sought to develop new biomaterials with fiber morphologies similar to the muscle fiber of the heart, but with surface energies similar to positive controls for cell attachment. Poly[2,3-bis-(3-octyloxyphenyl)quinoxaline-5,8-diyl-alt-thiophene-2,5-diyl] (TQ1) was then explored as a ribbon fiber and compared to collagen with embryonic cardiac cells, in vitro, and then implanted into rats for in vivo long term evaluations. The cardiac cells had a preferential adhesion to the TQ1 fibers, and in vivo, the fibers attracted more blood vessels and regrew functional tissue compared to the collagen controls. TQ1 fibers had the added ability to emit light in the near infrared region, which would allow for consistent tracking of the material. Although this material offered the morphological preference for the cardiac cells, it does not degrade and nor did it offer electrical conductivity. The heart muscle is an electrically active muscle. The dead tissue that is formed in the ischemic area loses its ability to  transfer the electrical signals. Hence, I have then developed collagen fibrous materials with silver nanowires to help store and inject charges that would be generated during the contraction of the heart muscle. The silver nanowires served to help carry charges whilst providing resistance to bacterial growth on the material. The collagen/silver nanowires composites were mechanically apt for the culture of embryonic cardiac cells.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2015. s. 80
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1686
Nationell ämneskategori
Fysik Klinisk medicin
Identifikatorer
urn:nbn:se:liu:diva-120773 (URN)10.3384/diss.diva-120773 (DOI)978-91-7519-021-1 (ISBN)
Disputation
2015-08-28, Planck, Fysikhuset, Campus Valla, Linköping, 09:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2015-08-24 Skapad: 2015-08-24 Senast uppdaterad: 2017-01-11Bibliografiskt granskad

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Wickham, Abeni M.Islam, Mohammad MirazulMondal, DebasishPhopase, JaywantSadhu, VeeraTamás, ÉvaPolisetti, NareshLiedberg, BoGriffith, May
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Molekylär fysikTekniska högskolanAvdelningen för cellbiologiHälsouniversitetetInstitutionen för klinisk och experimentell medicinInstitutionen för fysik, kemi och biologiTekniska fakultetenAvdelningen för kardiovaskulär medicinThorax-kärlkliniken i Östergötland
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Journal of Biomedical Materials Research. Part B - Applied biomaterials
Klinisk medicinMedicinska och farmaceutiska grundvetenskaperFysik

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