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Crime scene DNA sampling by wet-vacuum applying M-VAC
Swedish National Forensic Centre, Linköping, Sweden; Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden.
Swedish National Veterinary Institute, Uppsala, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten. Swedish National Forensic Centre, Linköping, Sweden.
2015 (Engelska)Ingår i: Forensic Science International: Genetics Supplement Series, ISSN 1875-1768, E-ISSN 1875-175X, Vol. 5, s. e89-e90Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A possible alternative to conventional stain recovery by swabbing, taping or cutting, is the M-Vac wet-vacuum instrument (M-Vac Systems Inc.). We have evaluated M-Vac for sampling of dried saliva on porous and non-porous surfaces, shed cells on clothes and touch DNA. M-Vac gave significantly higher DNA yields for dried saliva stains on laminated wood, compared with cotton swabs (average DNA concentrations 1.14 vs. 0.57 ng/μL, p = 0.02). For stains on glass, M-Vac and cotton swabs gave comparable DNA yields. Additionally, M-Vac retrieved three times as much DNA from saliva stains on cotton fabric (T-shirt) compared with saliva on towels (terry cloth), showing that the absorption properties of the surface affect wet-vacuum sampling. M-Vac was also applied for retrieving wearer DNA from clothes, enabling generation of complete DNA profiles from denim jeans, leggings and cotton T-shirt. A mixed DNA profile was retrieved from an “aggressor” pressing a hand against the shoulder area of a worn T-shirt. Since the major component of the obtained mixed DNA profile was from the wearer, M-Vac may not be ideal for touch DNA sampling of clothes. Wet-vacuum sampling requires a fairly large instrument, trained users and DNA extraction procedures handling large sample volumes. The complexity of M-Vac sampling prevents it from being extensively used, but in specific and important cases it can be a valuable sampling tool.

Ort, förlag, år, upplaga, sidor
Elsevier, 2015. Vol. 5, s. e89-e90
Nyckelord [en]
Cotton swab, Forensic DNA analysis, Saliva, Touch DNA
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:liu:diva-123671DOI: 10.1016/j.fsigss.2015.09.036OAI: oai:DiVA.org:liu-123671DiVA, id: diva2:891647
Tillgänglig från: 2016-01-07 Skapad: 2016-01-07 Senast uppdaterad: 2017-12-01Bibliografiskt granskad

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