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Segmentation of clustered nuclei based on concave curve expansion
North Ryde, New South Wales 2113, Australia.
The University of New South Wales, Canberra,Australia.
‡Aizu Research Cluster for Medical Engineering and Informatics, Research Center for AdvancedInformation Science and Technology, The University of Aizu, Fukushima, Japan.ORCID-id: 0000-0002-4255-5130
2013 (Engelska)Ingår i: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, ISSN 1365-2818, Vol. 251, nr 1, s. 57-67Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

Segmentation of nuclei from images of tissue sections is important for many biological and biomedical studies. Many existing image segmentation algorithms may lead to oversegmentation or undersegmentation for clustered nuclei images. In this paper, we proposed a new image segmentation algorithm based on concave curve expansion to correctly and accurately extract markers from the original images. Marker-controlled watershed is then used to segment the clustered nuclei. The algorithm was tested on both synthetic and real images and better results are achieved compared with some other state-of-the-art methods.

Ort, förlag, år, upplaga, sidor
2013. Vol. 251, nr 1, s. 57-67
Nyckelord [en]
clustered nuclei, concave curve expansion, curvature information, image segmentation, watershed segmentation
Nationell ämneskategori
Medicinsk bildbehandling
Identifikatorer
URN: urn:nbn:se:liu:diva-127921DOI: 10.1111/jmi.12043PubMedID: 23692597OAI: oai:DiVA.org:liu-127921DiVA, id: diva2:927945
Tillgänglig från: 2016-05-13 Skapad: 2016-05-13 Senast uppdaterad: 2017-11-30

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