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Author:
Carlsson, Jenny (Linköping University, Department of Physics, Chemistry and Biology, Applied Physics ) (Linköping University, The Institute of Technology)
Gullstrand, Camilla (Linköping University, Department of Clinical and Experimental Medicine, Pediatrics ) (Linköping University, Faculty of Health Sciences)
Westermark, Gunilla (Linköping University, Department of Clinical and Experimental Medicine, Cell Biology) (Linköping University, Faculty of Health Sciences)
Ludvigsson, Johnny (Linköping University, Department of Clinical and Experimental Medicine, Pediatrics ) (Linköping University, Faculty of Health Sciences) (Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping)
Enander, Karin (Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics ) (Linköping University, The Institute of Technology)
Liedberg, Bo (Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics ) (Linköping University, The Institute of Technology)
Title:
An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance
Department:
Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology
Linköping University, Faculty of Health Sciences
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics
Linköping University, The Institute of Technology
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics
Publication type:
Article in journal (Refereed)
Language:
English
Status:
Published
In:
Biosensors and Bioelectronics, ISSN 0956-5663
Volume:
24
Issue:
4
Pages:
876-881
Year of publ.:
2008
URI:
urn:nbn:se:liu:diva-12469
Permanent link:
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-12469
Subject category:
Other Basic Medicine
SVEP category:
Chemistry
Keywords(en) :
SPR, Type 1 diabetes, Insulin autoantibodies, Indirect competitive immunoassay, RIA
Abstract(en) :

We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA.

Note:
The status of article IV on the day of defence was: Accepted.
Available from:
2008-09-06
Created:
2008-09-06
Last updated:
2011-01-28
Statistics:
88 hits
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