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Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.
Department of Physiology, Hirosaki University School of Medicine, Hirosaki, Japan.
Department of Physiological Sciences, Lund University, Lund, Sweden.
Department of Physiological Sciences, Lund University, Lund, Sweden.
Department of Physiological Sciences, Lund University, Lund, Sweden.
Vise andre og tillknytning
2004 (engelsk)Inngår i: Methods, ISSN 1046-2023, E-ISSN 1095-9130, Vol. 33, nr 4, s. 302-311Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.

sted, utgiver, år, opplag, sider
Academic Press, 2004. Vol. 33, nr 4, s. 302-311
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Identifikatorer
URN: urn:nbn:se:liu:diva-133057DOI: 10.1016/j.ymeth.2004.01.003ISI: 000222459900007PubMedID: 15183179Scopus ID: 2-s2.0-2442717004OAI: oai:DiVA.org:liu-133057DiVA, id: diva2:1054568
Tilgjengelig fra: 2016-12-08 Laget: 2016-12-08 Sist oppdatert: 2018-01-13bibliografisk kontrollert

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