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Doping Polypyrrole Films with 4-N-Pentylphenylboronic Acid to Enhance Affinity towards Bacteria and Dopamine
Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Cranfield University, England.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Dundee, Scotland.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0003-3899-4891
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2016 (Engelska)Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, nr 11, artikel-id e0166548Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Here we demonstrate the use of a functional dopant as a fast and simple way to tune the chemical affinity and selectivity of polypyrrole films. More specifically, a boronic-functionalised dopant, 4-N-Pentylphenylboronic Acid (PBA), was used to provide to polypyrrole films with enhanced affinity towards diols. In order to prove the proposed concept, two model systems were explored: (i) the capture and the electrochemical detection of dopamine and (ii) the adhesion of bacteria onto surfaces. The chemisensor, based on overoxidised polypyrrole boronic doped film, was shown to have the ability to capture and retain dopamine, thus improving its detection; furthermore the chemisensor showed better sensitivity in comparison with overoxidised perchlorate doped films. The adhesion of bacteria, Deinococcus proteolyticus, Escherichia coli, Streptococcus pneumoniae and Klebsiella pneumoniae, onto the boric doped polypyrrole film was also tested. The presence of the boronic group in the polypyrrole film was shown to favour the adhesion of sugar-rich bacterial cells when compared with a control film (Dodecyl benzenesulfonate (DBS) doped film) with similar morphological and physical properties. The presented single step synthesis approach is simple and fast, does not require the development and synthesis of functional monomers, and can be easily expanded to the electrochemical, and possibly chemical, fabrication of novel functional surfaces and interfaces with inherent pre-defined sensing and chemical properties.

Ort, förlag, år, upplaga, sidor
PUBLIC LIBRARY SCIENCE , 2016. Vol. 11, nr 11, artikel-id e0166548
Nationell ämneskategori
Annan kemi
Identifikatorer
URN: urn:nbn:se:liu:diva-133388DOI: 10.1371/journal.pone.0166548ISI: 000388886000017PubMedID: 27875555OAI: oai:DiVA.org:liu-133388DiVA, id: diva2:1060070
Anmärkning

Funding Agencies|Ministry of Science Research and Technology of Iran; Linkoping University; Swedish Research Council [VR-2014-3079]; Erasmus exchange program of the European Commission

Tillgänglig från: 2016-12-27 Skapad: 2016-12-22 Senast uppdaterad: 2019-10-09
Ingår i avhandling
1. Functionalised surfaces for bacterial discrimination
Öppna denna publikation i ny flik eller fönster >>Functionalised surfaces for bacterial discrimination
2016 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Bacterial detection and identification is a critical step in many arenas, including food and water safety, clinical diagnostics, bioprocess control and biosecurity. Social hygiene has a direct correlation with the strict control of microorganisms in these fields. The worldwide cases of bacterial infectious disease is assessed to be 1-2 billion annually, and these have a massive negative effect on the global economy. Although many precise techniques are currently available, a huge mortality and morbidity related to bacterial infection disease continues to be reported annually due to misdiagnosis or delay in diagnosis. Increasing efficiency and reliability of pathogen detection methods will potentially improve social health and protect society against pathogenic diseases.

The development of culture media for selective isolation and differentiation of bacteria started in the late 19th century. Immunological assays and then genotyping techniques were developed in 20th century, in addition to many less commonly used techniques for bacterial detection. Each of the currently used methods has its advantages and weaknesses in terms of speed, cost and accuracy. Much effort has recently been devoted to developing biosensors for bacterial detection for simpler and more rapid use.

This thesis is focused on functionalised surfaces for the development of biosensors for bacterial discrimination and detection, and is divided in three subsections. First, we explored a new approach for bacterial discrimination based on pattern recognition. Traditional culturing methods discriminate bacteria based on their metabolic activity pattern. Taking inspiration from the extensive body of work that reports the use of electronic-noses to differentiate bacteria based on the volatiles patterns they produce, we explored the possibility of bacteria differentiation based on adhesion patterns. By altering the electropolymerisation conditions, the physicalchemical surface properties of polypyrrole (PPy) can be tuned to fabricate a range of dissimilar surfaces. The adhesion of different bacteria on a series of polymers was measured. Data analysis of the adhesion patterns proved that bacteria can be discriminated by examining their adhesion to dissimilar surfaces. Next, we developed a new functionalisation of PPy by doping PPy with 4-N-Pentylphenylboronic Acid and investigated the modulation of bacteria binding to those surfaces. In this second section, a new electropolymerisation technique for whole-cell imprinting was developed based on different functional monomers. 3-Aminophenyl boronic acid was shown to be a good monomer to produce whole-cell imprinted polymers (CIP) with high affinity for bacterial cells with improved releasing ability. Finally, in the third section aptamers, which are promising synthetic recognition elements, were explored for bacterial detection testing. A specific aptamer was used to fabricate of a prototype of label-free aptasensor for bacterial detection. Also, the SELEX process was used to produce a pool of aptamers, or “polyclonal” aptamers, which targeted a group of bacteria species. Using polyclonal aptamers as a recognition element enables biosensors to enhance their resolution to detect broader types of bacterial species using a single serological-like test.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2016. s. 55
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1770
Nyckelord
Biosensorer, Bakterier, Mönsterigenkänning
Nationell ämneskategori
Biomaterial
Identifikatorer
urn:nbn:se:liu:diva-160803 (URN)9789176857519 (ISBN)
Disputation
2016-06-17, Nobel, B-huset, Campus Valla, Linköping, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2019-10-09 Skapad: 2019-10-09 Senast uppdaterad: 2019-10-09Bibliografiskt granskad

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