liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
An integrated dual functional recognition/amplification bio-label for the one-step impedimetric detection of Micro-RNA-21
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0003-3274-6029
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Iranian National Institute Oceanog and Atmospher Science, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
Show others and affiliations
2017 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 92, p. 154-161Article in journal (Refereed) Published
Abstract [en]

Alteration in expression of miRNAs has been correlated with different cancer types, tumour stage and response to treatments. In this context, a structurally responsive oligonucleotide-based electrochemical impedimetric biosensor has been developed for the simple and sensitive detection of miRNA-21. A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles (AuNPs) to create an integrated, dual function bio-label (biotin-MB-AuNPs) for both biorecognition and signal generation. In the presence of target miRNA-21, hybridisation takes place resulting in the "activation" of the biotin-MB; this event makes the biotin group, which was previously "protected" by the steric hindrance of the MB stem-loop structure, accessible. The activated biotin-MB-AuNPs/miRNA complexes become available for capture, via supramolecular interaction, onto a nentravidin-modified electrode for electrochemical transduction. The binding event results in a decrease of the charge transfer resistance at the working electrode/electrolyte interface. The biosensor responded linearly in the range 1-1000 pM of miRNA-21, with a limit of detection of 0.3 pM, good reproducibility (Relative Standard deviation (RSD) =3.3%) and high selectivity over other miRNAs (i.e. miRNA221 and miRNA-205) sequences. Detection of miRNA-21 in spiked serum samples at clinically relevant levels (low pM range) was also demonstrated, thus illustrating the potential of the biosensor for point-of-care clinical applications. The proposed biosensor design, based on the combination of a neutravidin transducing surface and the dual-function biotin-MB-AuNPs bio-label, provides a simple and robust approach for detection of short-length nucleic acid targets, such as miRNAs.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2017. Vol. 92, p. 154-161
Keywords [en]
MicroRNA-21; Gold nanoparticles; Molecular beacon; Electrochemical impedance spectroscopy
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-136850DOI: 10.1016/j.bios.2017.02.014ISI: 000396946600023PubMedID: 28213328OAI: oai:DiVA.org:liu-136850DiVA, id: diva2:1092119
Note

Funding Agencies|European Communities Seventh Framework Program [PIRSES-GA-2012-318053]

Available from: 2017-04-30 Created: 2017-04-30 Last updated: 2018-03-28

Open Access in DiVA

fulltext(1001 kB)0 downloads
File information
File name FULLTEXT02.pdfFile size 1001 kBChecksum SHA-512
a14ab1e17fa8cd884c7714a7a751d2e368b9232bcda5dfba4b4e67684f8265d73f59bebd4ebfbe573c9b45ed50452cdf5cce5d4f7a7ab956fee088aca7ccfe41
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Mak, Wing CheungTurner, AnthonyBeni, Valerio
By organisation
Biosensors and BioelectronicsFaculty of Science & EngineeringDepartment of Physics, Chemistry and Biology
In the same journal
Biosensors & bioelectronics
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 270 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf