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Neutravidin biosensor for direct capture of dual-functional biotin-molecular beacon-AuNP probe for sensitive voltammetric detection of microRNA
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0002-1815-9699
University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
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2017 (engelsk)Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 248, s. 8s. 77-84Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We have demonstrated a new approach using a neutravidin-based biosensor combined with a dual-function gold nanoparticle (AuNP) biolabel, for simple and sensitive detection of microRNA-21 (miRNA-21). The selectivity of the biosensor is provided by the intrinsic properties of the dual-functional biotin-MB-AuNP label. The assay procedure is relatively simple, exploiting a one-pot assay concept where the affinity capture of the miRNA-21/dual-functional biotin-MB-AuNP complex, via the strong biotin-neutravidin supramolecular interaction, and simultaneous detection of the captured AuNPs label with stripping voltammetry, is performed in a single step. This electrochemical miRNA biosensor could detect miRNA-21 with limit of detection of 0.1×10less thansuperscriptgreater than−12less than/superscriptgreater than and a dynamic range from 0.5×10less thansuperscriptgreater than−12less than/superscriptgreater than to 1.0×10less thansuperscriptgreater than−9less than/superscriptgreater thanM. The performance of the miRNA-21biosensor was further improved after silver deposition onto the AuNPs, delivering an enhanced detection limit of 4.0×10less thansuperscriptgreater than−15less than/superscriptgreater thanM of miRNA-21, and an extremely wide analytic dynamic range from 10×10less thansuperscriptgreater than−15less than/superscriptgreater than to 1×10less thansuperscriptgreater than−9less than/superscriptgreater thanM (5 orders of magnitude). This exceptionally broad dynamic range demonstrates the advantage of the one-pot assay approach with direct capture of the dual functional biotin-MB-AuNP via the strong biotin-neutravidin supramolecular interaction. Furthermore, we demonstrated the detection of miRNA-21 in spiked serum at clinically relevant concentrations. The miRNA biosensor displayed excellent analytical performance for the detection of miRNA and could provide a powerful and convenient tool for biomedical research and applications in cancer diagnostics.

sted, utgiver, år, opplag, sider
Elsevier, 2017. Vol. 248, s. 8s. 77-84
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URN: urn:nbn:se:liu:diva-137576DOI: 10.1016/j.snb.2017.03.160ISI: 000403772200011OAI: oai:DiVA.org:liu-137576DiVA, id: diva2:1097104
Merknad

Funding agencies: SMARTCANCERSENS project from the European Communities Seventh Framework Program [PIRSES-GA-2012-318053]

Tilgjengelig fra: 2017-05-22 Laget: 2017-05-22 Sist oppdatert: 2018-03-19

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