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Enhanced biomethane production rate and yield from lignocellulosic ensiled forage ley by in situ anaerobic digestion treatment with endogenous cellulolytic enzymes
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten. Karshult Municipal Waste Water Treatment Plant, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten. InZymes Biotech AB, Gjuterigatan 1B, S-58273 Linkoping, Sweden.
2017 (engelsk)Inngår i: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 10, artikkel-id 129Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Enzymatic treatment of lignocellulosic material for increased biogas production has so far focused on pretreatment methods. However, often combinations of enzymes and different physicochemical treatments are necessary to achieve a desired effect. This need for additional energy and chemicals compromises the rationale of using enzymes for low energy treatment to promote biogas production. Therefore, simpler and less energy intensive in situ anaerobic digester treatment with enzymes is desirable. However, investigations in which exogenous enzymes are added to treat the material in situ have shown mixed success, possibly because the enzymes used originated from organisms not evolutionarily adapted to the environment of anaerobic digesters. In this study, to examine the effect of enzymes endogenous to methanogenic microbial communities, cellulolytic enzymes were instead overproduced and collected from a dedicated methanogenic microbial community. By this approach, a solution with very high endogenous microbial cellulolytic activity was produced and tested for the effect on biogas production from lignocellulose by in situ anaerobic digester treatment. Results: Addition of enzymes, endogenous to the environment of a mixed methanogenic microbial community, to the anaerobic digestion of ensiled forage ley resulted in significantly increased rate and yield of biomethane production. The enzyme solution had an instant effect on more readily available cellulosic material. More importantly, the induced enzyme solution also affected the biogas production rate from less accessible cellulosic material in a second slower phase of lignocellulose digestion. Notably, this effect was maintained throughout the experiment to completely digested lignocellulosic substrate. Conclusions: The induced enzyme solution collected from a microbial methanogenic community contained enzymes that were apparently active and stable in the environment of anaerobic digestion. The enzymatic activity had a profound effect on the biogas production rate and yield, comparable with the results of many pretreatment methods. Thus, application of such enzymes could enable efficient low energy in situ anaerobic digester treatment for increased biomethane production from lignocellulosic material.

sted, utgiver, år, opplag, sider
BIOMED CENTRAL LTD , 2017. Vol. 10, artikkel-id 129
Emneord [en]
Biogas; Lignocellulose; Cellulolytic; Cellulase; Enzyme; Hydrolysis; Biochemical methane potential (BMP); Anaerobic digestion; In situ
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-138248DOI: 10.1186/s13068-017-0814-0ISI: 000401621100002PubMedID: 28523077OAI: oai:DiVA.org:liu-138248DiVA, id: diva2:1109377
Merknad

Funding Agencies|Swedish Research Council [621-2009-4150]; Swedish Energy Agency through the Biogas Research Center [P33609-1]

Tilgjengelig fra: 2017-06-14 Laget: 2017-06-14 Sist oppdatert: 2018-05-07
Inngår i avhandling
1. Influencing anaerobic digestion early stage processes for increased biomethane production from different substrate components
Åpne denne publikasjonen i ny fane eller vindu >>Influencing anaerobic digestion early stage processes for increased biomethane production from different substrate components
2018 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Finding alternatives to petroleum-based energy sources is of interest since it could reduce the emissions of net carbon dioxide to the atmosphere by increasing the usage of renewable energy sources. To do so improvements are needed in the renewable energy production sector. Biogas production is of interest since the anaerobic digestion process can degrade many different biomolecules and is, contrary to e.g. bioethanol and biodiesel, not dependent on specific molecules. Thus, many wastes such as slaughterhouse waste, sludge from waste water treatment and lignocellulose residual material etc. can be used as substrates for biogas production. However, there are limitations in the degradation process depending on the composition of the selected substrate. To overcome these limitations such as inhibition of different microorganisms, or recalcitrant substrate, different methods can be used to increase the biogas production.

 In this study different substrates were selected and analyzed/treated for remedies of early stage rate limiting problems of the anaerobic digestion process. Different analyzes and techniques were selected depending on the limitations correlated to the main problematic component of the specific substrate.

 Improvements could be reached for the degradation of slaughterhouse waste by augmentation with the clay mineral zeolite. Addition of different enzymes to the substrate environment of different waste water treatment plant sludges resulted in limited life time of the selected enzymes. However, certain enzymes proved to be promising candidates with an effect of increased biogas production rate and yield for the time that the enzyme remained active. In an additional experiment, cellulolytic enzymes, naturally produced by a biogas producing microbial community, were induced, collected and added to a biogas experiment of ensiled forage ley, by which it was shown that these cellulases led to an increase in biogas production rate and yield. Thus, the studies demonstrate different techniques for improving the anaerobic digestion process of different types of substrates.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2018. s. 56
Serie
Linköping Studies in Science and Technology. Licentiate Thesis, ISSN 0280-7971 ; 1807
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-147721 (URN)10.3384/lic.diva-147721 (DOI)9789176852866 (ISBN)
Presentation
2018-05-31, BL21 (Nobel), Campus Valla, Linköping, 10:30 (engelsk)
Opponent
Merknad

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Tilgjengelig fra: 2018-05-07 Laget: 2018-05-07 Sist oppdatert: 2019-10-12bibliografisk kontrollert

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