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Discovering viral genomes in human metagenomic data by predicting unknown protein families
Karolinska Inst, Sweden.
Stockholm Univ, Sweden.
Karolinska Inst, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Bioinformatik. Linköpings universitet, Tekniska fakulteten.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, artikkel-id 28Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Massive amounts of metagenomics data are currently being produced, and in all such projects a sizeable fraction of the resulting data shows no or little homology to known sequences. It is likely that this fraction contains novel viruses, but identification is challenging since they frequently lack homology to known viruses. To overcome this problem, we developed a strategy to detect ORFan protein families in shotgun metagenomics data, using similarity-based clustering and a set of filters to extract bona fide protein families. We applied this method to 17 virus-enriched libraries originating from human nasopharyngeal aspirates, serum, feces, and cerebrospinal fluid samples. This resulted in 32 predicted putative novel gene families. Some families showed detectable homology to sequences in metagenomics datasets and protein databases after reannotation. Notably, one predicted family matches an ORF from the highly variable Torque Teno virus (TTV). Furthermore, follow-up from a predicted ORFan resulted in the complete reconstruction of a novel circular genome. Its organisation suggests that it most likely corresponds to a novel bacteriophage in the microviridae family, hence it was named bacteriophage HFM.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP , 2018. Vol. 8, artikkel-id 28
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-144887DOI: 10.1038/s41598-017-18341-7ISI: 000419441300028PubMedID: 29311716OAI: oai:DiVA.org:liu-144887DiVA, id: diva2:1181673
Merknad

Funding Agencies|Swedish Research Council; Knut and Alice Wallenberg Foundation

Tilgjengelig fra: 2018-02-09 Laget: 2018-02-09 Sist oppdatert: 2018-03-05

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