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A clip-on electroosmotic pump for oscillating flow in microfluidic cell culture devices
Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska fakulteten. LunaMicro AB, Linköping, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0001-5914-2837
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0001-9711-794X
Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska fakulteten. LunaMicro AB, Linköping, Sweden.ORCID-id: 0000-0002-2773-5096
2018 (engelsk)Inngår i: Microfluidics and Nanofluidics, ISSN 1613-4982, E-ISSN 1613-4990, Vol. 22, nr 3, artikkel-id 27Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Recent advances in microfluidic devices put a high demand on small, robust and reliable pumps suitable for high-throughput applications. Here we demonstrate a compact, low-cost, directly attachable (clip-on) electroosmotic pump that couples with standard Luer connectors on a microfluidic device. The pump is easy to make and consists of a porous polycarbonate membrane and poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. The soft electrode and membrane materials make it possible to incorporate the pump into a standard syringe filter holder, which in turn can be attached to commercial chips. The pump is less than half the size of the microscope slide used for many commercial lab-on-a-chip devices, meaning that these pumps can be used to control fluid flow in individual reactors in highly parallelized chemistry and biology experiments. Flow rates at various electric current and device dimensions are reported. We demonstrate the feasibility and safety of the pump for biological experiments by exposing endothelial cells to oscillating shear stress (up to 5 dyn/cm2) and by controlling the movement of both micro- and macroparticles, generating steady or oscillatory flow rates up to ± 400 μL/min.

sted, utgiver, år, opplag, sider
Springer Berlin/Heidelberg, 2018. Vol. 22, nr 3, artikkel-id 27
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-145301DOI: 10.1007/s10404-018-2046-4ISI: 000427527600005OAI: oai:DiVA.org:liu-145301DiVA, id: diva2:1184492
Merknad

Funding agencies: Swedish Research Council (Vetenskapsradet) [2015-03298]

Tilgjengelig fra: 2018-02-21 Laget: 2018-02-21 Sist oppdatert: 2019-01-22bibliografisk kontrollert
Inngår i avhandling
1. Organs-on-chips for the pharmaceutical development process: design perspectives and implementations
Åpne denne publikasjonen i ny fane eller vindu >>Organs-on-chips for the pharmaceutical development process: design perspectives and implementations
2018 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Organs-on-chips are dynamic cell culture devices created with the intention to mimic organ function in vitro. Their purpose is to assess the toxicity and efficacy of drugs and, as early as possible in the pharmaceutical development process, predict the outcome of clinical trials. The aim of this thesis is to explain and discuss these cell culture devices from a design perspective and to experimentally exemplify some of the specific functions that characterize organs-on-chips.

The cells in our body reside in complex environments with chemical and mechanical cues that affect their function and purpose. Such a complex environment is difficult to recreate in the laboratory and has therefore been overlooked in favor of more simple models, i.e. static twodimensional (2D) cell cultures. Numerous recent reports have shown cell culture systems that can resemble the cell’s natural habitat and enhance cell functionality and thereby potentially provide results that better reflects animal and human trials. The way these organs-on-chips improve in vitro cell culture assays is to include e.g. a three-dimensional cell architecture (3D), mechanical stimuli, gradients of oxygen or nutrients, or by combining several relevant cell types that affect each other in close proximity.

The research conducted for this thesis shows how cells in 3D spheroids or in 3D hydrogels can be cultured in perfused microbioreactors. Furthermore, a pump based on electroosmosis, and a method for an objective conceptual design process, is introduced to the field of organs-on-chips.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2018. s. 78
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1907
Emneord
Organs-on-chips, cell culture models, pharmaceutical development, microfluidics
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-145300 (URN)10.3384/diss.diva-145300 (DOI)9789176853597 (ISBN)
Disputas
2018-03-23, Planck, Fysikhuset, Campus Valla, Linköping, 13:30 (engelsk)
Opponent
Veileder
Merknad

I den tryckta versionen är det ena serienamnet felaktigt. I den elektroniska versionen är detta ändrat till korrekt "Linköping Studies in Science and Technology. Dissertations"

Tilgjengelig fra: 2018-02-21 Laget: 2018-02-21 Sist oppdatert: 2019-09-26bibliografisk kontrollert

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