Proteomic Analyses of the Unexplored Sea Anemone Bunodactis verrucosaShow others and affiliations
2018 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 16, no 2, article id 42Article in journal (Refereed) Published
Abstract [en]
Cnidarian toxic products, particularly peptide toxins, constitute a promising target for biomedicine research. Indeed, cnidarians are considered as the largest phylum of generally toxic animals. However, research on peptides and toxins of sea anemones is still limited. Moreover, most of the toxins from sea anemones have been discovered by classical purification approaches. Recently, high-throughput methodologies have been used for this purpose but in other Phyla. Hence, the present work was focused on the proteomic analyses of whole-body extract from the unexplored sea anemone Bunodactis verrucosa. The proteomic analyses applied were based on two methods: two-dimensional gel electrophoresis combined with MALDI-TOF/TOF and shotgun proteomic approach. In total, 413 proteins were identified, but only eight proteins were identified from gel-based analyses. Such proteins are mainly involved in basal metabolism and biosynthesis of antibiotics as the most relevant pathways. In addition, some putative toxins including metalloproteinases and neurotoxins were also identified. These findings reinforce the significance of the production of antimicrobial compounds and toxins by sea anemones, which play a significant role in defense and feeding. In general, the present study provides the first proteome map of the sea anemone B. verrucosa stablishing a reference for future studies in the discovery of new compounds.
Place, publisher, year, edition, pages
MDPI , 2018. Vol. 16, no 2, article id 42
Keywords [en]
cnidarian; sea anemone; proteins; toxins; two-dimensional gel electrophoresis; MALDI-TOF; TOF; shotgun proteomic
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-147249DOI: 10.3390/md16020042ISI: 000427528800004PubMedID: 29364843OAI: oai:DiVA.org:liu-147249DiVA, id: diva2:1197202
Note
Funding Agencies|Portuguese Foundation for Science and Technology (FCTFundacAo para a Ciencia e a Tecnologia, Portugal) [SFRH/BD/80592/2011]; FCT [SFRH/BPD/92978/2013, SFRH/BPD/103683/2014]; Alexander von Humboldt postdoctoral fellowship (Collaborative Research Centre -German Research Foundation) [3.2-KUB/1153731, 1279]; FCT and the European Regional Development Fund (ERDF) [UID/Multi/04423/2013]; European Structural and Investment Funds (ESIF) through the Competitiveness and Internationalization Operational ProgramCOMPETE 2020; National Funds through the FCT [PTDC/AAG-GLO/6887/2014, (POCI-01-0124-FEDER-016845)]; Structured Programs of R&D&I INNOVMARInnovation and Sustainability in the Management and Exploitation of Marine Resources [NORTE-01-0145-FEDER-000035]; CORAL NORTE [NORTE-01-0145-FEDER-000036]; Northern Regional Operational Program (NORTE) through the ERDF
2018-04-122018-04-122023-10-12