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Fabrication of Protein Microparticles and Microcapsules with Biomolecular Tools
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Hong Kong Univ Sci and Technol, Peoples R China.
Linköping University, Department of Physics, Chemistry and Biology, Sensor and Actuator Systems. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0003-3274-6029
2018 (English)In: Zeitschrift fur physikalische Chemie (Munchen. 1991), ISSN 0942-9352, Vol. 232, no 5-6, p. 759-771Article in journal (Refereed) Published
Abstract [en]

Microparticles have attracted much attention for medical, analytical and biological applications. Calcium carbonate (CaCO3) templating method with the advantages of having narrow size distribution, controlled morphology and good biocompatibility that has been widely used for the synthesis of various protein-based microparticles. Despite CaCO3 template is biocompatible, most of the conventional methods to create stable protein microparticles are mainly driven by chemical crosslink reagents which may induce potential harmful effect and remains undesirable especially for biomedical or clinical applications. In this article, we demonstrate the fabrication of protein microparticles and microcapsules with an innovative method using biomolecular tools such as enzymes and affinity molecules to trigger the assembling of protein molecules within a porous CaCO3 template followed by a template removal step. We demonstrated the enzyme-assisted fabrication of collagen microparticles triggered by transglutaminase, as well as the affinity-assisted fabrication of BSA-biotin avidin microcapsules triggered by biotin-avidin affinity interaction, respectively. Based on the different protein assemble mechanisms, the collagen microparticles appeared as a solid-structured particles, while the BSA-biotin avidin microcapsules appeared as hollow-structured morphology. The fabrication procedures are simple and robust that allows producing protein microparticles or microcapsules under mild conditions at physiological pH and temperature. In addition, the microparticle morphologies, protein compositions and the assemble mechanisms were studied. Our technology provides a facile approach to design and fabricate protein microparticles and microcapsules that are useful in the area of biomaterials, pharmaceuticals and analytical chemistry.

Place, publisher, year, edition, pages
WALTER DE GRUYTER GMBH , 2018. Vol. 232, no 5-6, p. 759-771
Keywords [en]
affinity interaction; enzymatic reaction; microcapsules; microparticles; protein
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-149381DOI: 10.1515/zpch-2017-1052ISI: 000434485200011OAI: oai:DiVA.org:liu-149381DiVA, id: diva2:1229731
Available from: 2018-07-02 Created: 2018-07-02 Last updated: 2018-07-02

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Cheung, KittMak, Wing Cheung
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Zeitschrift fur physikalische Chemie (Munchen. 1991)
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