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Effect of length of time post-mortem on quality and freezing capacity of Cantabric chamois (Rupicapra pyrenaica parva) epididymal spermatozoa
Linköping University, Department of Clinical and Experimental Medicine, Division of Children's and Women's health. Linköping University, Faculty of Medicine and Health Sciences. Univ Autonoma Barcelona, Spain.
Univ Leon, Spain.
Univ Leon, Spain.
Univ Leon, Spain.
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2018 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 198, p. 184-192Article in journal (Refereed) Published
Abstract [en]

Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at - 20 degrees C/min). Sperm quality was assessed after recovery and post-thawing (motility by CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). The sperm mass pH and osmolality showed a positive correlation with time. Total sperm motility dropped after 2 days PM, with progressivity and sperm velocities remained similar up to 3 days PM. Sperm freezability was acceptable, with the post-thawing HOST, motility, progressivity, VAP, VCL, VSL and BCF negatively correlating with PM time. Overall, chamois epidydimal samples were not adequate for preservation after 6 days PM. Freezability capacity could make these spermatozoa suitable for specific ART even if kept refrigerated for several days PM.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 198, p. 184-192
Keywords [en]
Cantabric chamois; Cryopreservation; Epididymal sperm; Post-mortem time; Germplasm banking
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-152807DOI: 10.1016/j.anireprosci.2018.09.018ISI: 000449567400022PubMedID: 30301620OAI: oai:DiVA.org:liu-152807DiVA, id: diva2:1265290
Note

Funding Agencies|Ministry of Education, Culture and Sports, Spain; Ramon y Cajal programme (Spanish Ministry of Science and Innovation)

Available from: 2018-11-22 Created: 2018-11-22 Last updated: 2018-11-22

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Alvarez-Rodriguez, Manuel
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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