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Generic Neutravidin Biosensor for Simultaneous Multiplex Detection of MicroRNAs via Electrochemically Encoded Responsive Nanolabels
Not Found:Linkoping Univ, Biosensors and Bioelect Ctr, Dept Phys Chem and Biol IFM, S-58183 Linkoping, Sweden; Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, Faculty of Science & Engineering. Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
Linköping University, Department of Physics, Chemistry and Biology, Sensor and Actuator Systems. Linköping University, Faculty of Science & Engineering. Cranfield Univ, England.ORCID iD: 0000-0002-1815-9699
Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
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2019 (English)In: ACS SENSORS, ISSN 2379-3694, Vol. 4, no 2, p. 326-334Article in journal (Refereed) Published
Abstract [en]

Current electrochemical biosensors for multiple miRNAs require tedious immobilization of various nucleic acid probes. Here, we demonstrate an innovative approach using a generic neutravidin biosensor combined with electrochemically encoded responsive nanolabels for facile and simultaneous multiplexed detection of miRNA-21 and miRNA-141. The selectivity of the biosensor arises from the intrinsic properties of the electrochemically encoded responsive nanolabels, comprising biotinylated molecular beacons (biotin-MB) and metal nanoparticles (metal-NPs). The procedure is a simple one-pot assay, where the targeted miRNA causes the opening of biotin-MB followed by capturing of the biotin-MB-metal-NPs by the neutravidin biosensor and simultaneous detection of the captured metal-NPs by stripping square-wave voltammetry (SSWV). The multiplexed detection of miRNA-21 and miRNA-141 is achieved by differentiation of the electrochemical signature (i.e., the peak current) for the different metal-NP labels. The biosensor delivers simultaneous detection of miRNAs with a linear range of 0.5-1000 pM for miRNA-21 and a limit of detection of 0.3 pM (3 sigma/sensitivity, n = 3), and a range of 50-1000 pM for miRNA-141, with a limit of detection of 10 pM. Furthermore, we demonstrate multiplexed detection of miRNA-21 and miRNA-141 in a spiked serum sample.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 4, no 2, p. 326-334
Keywords [en]
neutravidin biosensor; multiplex detection; microRNAs; molecular beacons; nanoparticle labels
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-155593DOI: 10.1021/acssensors.8b00942ISI: 000459836400009PubMedID: 30730699OAI: oai:DiVA.org:liu-155593DiVA, id: diva2:1297817
Note

Funding Agencies|EU-FP7 project [PIRSES-GA-2012-318053]

Available from: 2019-03-21 Created: 2019-03-21 Last updated: 2019-03-21

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