liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Optimization of Acidic Degradation of Hyaluronic Acid using Design of Experiments
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology.
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Hyaluronic acid (HA) is an unbranched polysaccharide consisting of the repeating disaccharide unit β(1→4)-GlcA-β(1→3)-GlcNAc and is a naturally occurring biopolymer in bacteria and vertebras. HA is predominantly found in the extracellular matrix (ECM) and the in vivo function of HA can vary depending on molecular weight (Mw) for instance high Mw HA is reported to be anti-angiogenic while low Mw HA induces angiogenesis. HA is a popular component for hydrogels such as dermal fillers. HA is commonly used in dermal fillers. However, other materials, such as other polymers, can be used as well. The project goal was to investigate different degradation processes for production of target Mw HA. Alkaline and acidic degradation processes in combination with increased temperatures seemed as the most promising methods. Degradation tests performed both in aqueous solution as well as heterogeneously in ethanol were evaluated. The acidic degradation in aqueous solution was proven to have the largest degradation constant. Both a robustness test as well as a Design of Experiments (DoE) was performed to investigate the influence different factors had on the degradation speed. The investigated factors were HA concentration, HCl concentration and temperature. Temperature and HCl concentrations proved to be the most influencing factors and a model was developed in the DoE software MODDE to describe how the factors influenced the degradation constant. The model was established as a good significant model with a Q2 value of 0.998 and relative standard deviation (RSD) value of 0.022 after a logarithmic transformation was performed as well as a simplification of the model by excluding some of the factor interactions. The acidic degradation method also proved to be a highly robust method which easily could be used to produce target Mw HA.

Place, publisher, year, edition, pages
2019. , p. 31
Keywords [en]
Hyaluronic Acid, Molecular Weight, Acidic Degradation, Heterogeneous Degradation, Robustness Testing, Degradation Constant, Design of Experiments, Degradation, DoE
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:liu:diva-156273ISRN: LITH-IFM-A-EX--19/3602--SEOAI: oai:DiVA.org:liu-156273DiVA, id: diva2:1303721
External cooperation
Galderma/Q-Med AB
Presentation
2019-03-04, Linköping, 13:00 (English)
Examiners
Available from: 2019-04-15 Created: 2019-04-10 Last updated: 2019-04-15Bibliographically approved

Open Access in DiVA

fulltext(1868 kB)19 downloads
File information
File name FULLTEXT01.pdfFile size 1868 kBChecksum SHA-512
b4446843ceaaf40089bd1dbc9d4791693b491301ecbdd0f6ed528d7aa37a7964c1a80f87a7e14eba82f5d214896f564f083323302effc419adb068011f7f0acd
Type fulltextMimetype application/pdf

By organisation
Biotechnology
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 19 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 43 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf