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The Effect of Enzymatic Digestion on Cultured Epithelial Autografts
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för Kirurgi, Ortopedi och Onkologi. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Sinnescentrum, Hand- och plastikkirurgiska kliniken US.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0002-7001-9415
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2019 (engelsk)Inngår i: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 28, nr 5, s. 638-644Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Severe burns are often treated by means of autologous skin grafts, preferably following early excision of the burnt tissue. In the case of, for example, a large surface trauma, autologous skin cells can be expanded in vitro prior to transplantation to facilitate the treatment when insufficient uninjured skin is a limitation. In this study we have analyzed the impact of the enzyme (trypsin or accutase) used for cell dissociation and the incubation time on cell viability and expansion potential, as well as expression of cell surface markers indicative of stemness. Skin was collected from five individuals undergoing abdominal reduction surgery and the epidermal compartment was digested in either trypsin or accutase. Trypsin generally generated more cells than accutase and with higher viability; however, after 7 days of subsequent culture, accutase-digested samples tended to have a higher cell count than trypsin, although the differences were not significant. No significant difference was found between the enzymes in median fluorescence intensity of the analyzed stem cell markers; however, accutase digestion generated significantly higher levels of CD117- and CD49f-positive cells, but only in the 5 h digestion group. In conclusion, digestion time appeared to affect the isolated cells more than the choice of enzyme.

sted, utgiver, år, opplag, sider
Sage Publications, 2019. Vol. 28, nr 5, s. 638-644
Emneord [en]
autografts, cell culture, epithelial cells, keratinocytes, stem cells
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-156422DOI: 10.1177/0963689719833305ISI: 000477971000013PubMedID: 30983404OAI: oai:DiVA.org:liu-156422DiVA, id: diva2:1306059
Merknad

Funding agencies: Integrative Regenerative Medicine (IGEN) center at Linkoping University

Tilgjengelig fra: 2019-04-23 Laget: 2019-04-23 Sist oppdatert: 2019-08-19

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