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DNA sampling using different tissues from the butterfly species Vanessa cardui
Linköping University, Department of Physics, Chemistry and Biology.
2019 (English)Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
Abstract [en]

The fundamental challenge to prevent species from going extinct is difficult but of grave importance. Halting species from going extinct minimizes the loss of biodiversity. One way of researching biodiversity is by studying species on a genetic level. This creates a dilemma as studying species genetically often requires using destructive sampling and is not desirable or even allowed when studying threatened species. Thus, there is a necessity for alternate sampling methods. In this study both non-lethal and lethal methods were used to gather tissues from the butterfly species Vanessa cardui. The DNA extractions turned out to give varying amounts of DNA, but it was successfully extracted from all the different tissue types. Amplifiable DNA was successfully gained using PCR and confirmed using gel electrophoresis. Existing and newly designed primers for multi-copy genes were used and several of them gave amplifiable DNA. Even if amplifiable DNA has been obtained in other studies, using various tissues, it turned out to only work with a live butterfly’s body in this study.

Place, publisher, year, edition, pages
2019. , p. 21
Keywords [en]
Vanessa cardui, DNA extraction, non-lethal methods, PCR, Gel electrophoresis, biodiversity
National Category
Natural Sciences Genetics
Identifiers
URN: urn:nbn:se:liu:diva-158611ISRN: LITH-IFM-G-EX--19/3699--SEOAI: oai:DiVA.org:liu-158611DiVA, id: diva2:1335131
Subject / course
Chemical Biology
Educational program
Teacher Education
Presentation
2019-05-29, BL 32, Linköping, 08:35 (Swedish)
Supervisors
Examiners
Available from: 2019-08-15 Created: 2019-07-03 Last updated: 2019-08-15Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf