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Determination of Fucose Concentration in a Lectin-Based Displacement Microfluidic Assay
Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten.ORCID-id: 0000-0002-2773-5096
2019 (engelsk)Inngår i: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 188, nr 3, s. 868-877Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We compare three different methods to quantify the monosaccharide fucose in solutions using the displacement of a large glycoprotein, lactoferrin. Two microfluidic analysis methods, namely fluorescence detection of (labeled) lactoferrin as it is displaced by unlabeled fucose and the displacement of (unlabeled) lactoferrin in SPR, provide fast responses and continuous data during the experiment, theoretically providing significant information regarding the interaction kinetics between the saccharide groups and binding sites. For comparison, we also performed a static displacement ELISA. The stationary binding site in all cases was immobilized S2-AAL, a monovalent polypeptide based on Aleuria aurantia lectin. Although all three assays showed a similar dynamic range, the microfluidic assays with fluorescent or SPR detection show an advantage in short analysis times. Furthermore, the microfluidic displacement assays provide a possibility to develop a one-step analytical platform.

sted, utgiver, år, opplag, sider
SPRINGER , 2019. Vol. 188, nr 3, s. 868-877
Emneord [en]
Competitive binding; Fucose; Surface plasmon resonance; Protein-carbohydrate interaction; Fluorescence detection; Microfluidic assay
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-158829DOI: 10.1007/s12010-018-02944-5ISI: 000471851400019PubMedID: 30710193OAI: oai:DiVA.org:liu-158829DiVA, id: diva2:1337684
Merknad

Funding Agencies|Health Research Council of Southeast of Sweden [FORSS-389021]; LIST grant from Linkoping University

Tilgjengelig fra: 2019-07-16 Laget: 2019-07-16 Sist oppdatert: 2019-11-07

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