liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Extracellular vesicles isolated from porcine seminal plasma exhibit different tetraspanin expression profiles
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Extremadura, Spain.
Vise andre og tillknytning
2019 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, artikkel-id 11584Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Seminal extracellular vesicles (EVs) include exosomes (phi 40-120 nm) and microvesicles (MVs, phi 120-1000 nm), which would be involved in multiple functional reproductive roles. The study aimed to establish which EV subtypes are present in pig semen, using a high-resolution flow cytometer to explore differences in their tetraspanin expression profile. The EVs were isolated from 12 pig ejaculates using serial ultracentrifugation and characterized by dynamic light scattering and electron microscopy for size and morphology as well as for tetraspanin expression using flow cytometry with Carboxyfluorescein succinimidyl ester (CFSE) and antibodies against CD9, CD63 and CD81. Pig semen contained a heterogeneous EV-population regarding size and morphology. Flow cytometric analysis demonstrated that the proportion of EVs expressing CD63 and CD9 was higher in MVs (P amp;lt; 0.001 and P amp;lt; 0.05, respectively) than in exosomes, while the opposite was true for CD81; higher (P amp;lt; 0.001) in exosomes than in MVs. In conclusion, (1) the new generation of flow cytometers are able to accurately identify EVs and to gate them in two size-different populations named exosomes and MVs. (2) Tetraspanins CD9, CD63 and CD81 are present in both seminal EVs, albeit with exosomes and MVs differing in expression profiles, suggesting dissimilar cargo and binding affinity.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP , 2019. Vol. 9, artikkel-id 11584
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-159861DOI: 10.1038/s41598-019-48095-3ISI: 000480233800036PubMedID: 31399634OAI: oai:DiVA.org:liu-159861DiVA, id: diva2:1346286
Merknad

Funding Agencies|MINECO Madrid (Spain) [AGL2015-69738-R]; FEDER Madrid (Spain) [AGL2015-69738-R]; Seneca Foundation Murcia (Spain) [19892/GERM/15]; Swedish Research Council Formas, Sweden [2017-00946]; Research Council in Southeast Sweden (FORSS), Sweden [745971]; MICINN (Madrid, Spain); MINECO (Madrid, Spain)

Tilgjengelig fra: 2019-08-27 Laget: 2019-08-27 Sist oppdatert: 2019-11-22

Open Access i DiVA

fulltext(2245 kB)6 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2245 kBChecksum SHA-512
58cb46fdbe619cb867cb913464af17796958d71d38fd8cc5615999fe6165bd090766daa3687240f5e387941cc436fc1a3daf9417e5d31d102e1301e88aa6b75b
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Søk i DiVA

Av forfatter/redaktør
Rodriguez-Martinez, Heriberto
Av organisasjonen
I samme tidsskrift
Scientific Reports

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 6 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 38 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf