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EGOFET Peptide Aptasensor for Label-Free Detection of Inflammatory Cytokines in Complex Fluids
Univ Modena and Reggio Emilia, Italy; Univ Ferrara, Italy.
Univ Modena and Reggio Emilia, Italy.
Univ Catania, Italy.
Univ Modena and Reggio Emilia, Italy.
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2018 (English)In: ADVANCED BIOSYSTEMS, ISSN 2366-7478, Vol. 2, no 2, article id 1700072Article in journal (Refereed) Published
Abstract [en]

Organic electronic transistors are rapidly emerging as ultrahigh sensitive label-free biosensors suited for point-of-care or in-field deployed applications. Most organic biosensors reported to date are based on immunorecognition between the relevant biomarkers and the immobilized antibodies, whose use is hindered by large dimensions, poor control of sequence, and relative instability. Here, an electrolyte-gated organic field effect transistor (EGOFET) biosensor where the recognition units are surface immobilized peptide aptamers (Affimer proteins) instead of antibodies is reported. Peptide aptasensor for the detection of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF alpha) with a 1 x 10(-12) M limit of detection is demonstrated. Ultralow sensitivity is met even in complex solutions such as cell culture media containing 10% serum, demonstrating the remarkable ligand specificity of the device. The device performances, together with the simple one-step immobilization strategy of the recognition moieties and the low operational voltages, all prompt EGOFET peptide aptasensors as candidates for early diagnostics and monitoring at the point-of-care.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2018. Vol. 2, no 2, article id 1700072
Keywords [en]
Affimer protein; binding constant; organic bioelectronics; peptide aptamers; TNF alpha
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-160145DOI: 10.1002/adbi.201700072ISI: 000446967100001OAI: oai:DiVA.org:liu-160145DiVA, id: diva2:1349346
Note

Funding Agencies|IT MIUR Bilateral Project Italy/Sweden "Poincare"; INFM-CNR Project "EOS - Organic electronics for innovative measuring instruments"; Life Science Department through "FAR"; "Fondazione di Vignola"

Available from: 2019-09-09 Created: 2019-09-09 Last updated: 2019-09-16

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Berggren, MagnusSimon, Daniel
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Laboratory of Organic ElectronicsFaculty of Science & Engineering
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