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Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering.
Linköping University, Faculty of Medicine and Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Division of Hematopoiesis and Developmental Biology.
Linköping University, Department of Physics, Chemistry and Biology, Sensor and Actuator Systems. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-2071-7768
2018 (English)In: Journal of Materials Chemistry B, ISSN 2050-750X, Vol. 6, p. 4665-4675Article in journal (Refereed) Published
Abstract [en]

Hematopoietic stem cells are used in transplantations for patients with hematologic malignancies. Scarce sources require efficient strategies of expansion, including polymeric biomaterials mimicking architectures of bone marrow tissue. Tissue microenvironment and mode of cytokine presentation strongly influence cell fate. Although several cytokines with different functions as soluble or membrane-bound mediators have already been identified, their precise roles have not yet been clarified. A need exists for in vitro systems that mimic the in vivo situation to enable such studies. One way is to establish surfaces mimicking physiological presentation using protein-immobilization onto polymer films. However these films merely provide a static presentation of the immobilized proteins. It would be advantageous to also dynamically change protein presentation and functionality to better reflect the in vivo conditions. The electroactive polymer polypyrrole shows excellent biocompatibility and electrochemically alters its surface properties, becoming an interesting choice for such setups. Here, we present an in vitro system for switchable presentation of membrane-bound cytokines. We use interleukin IL-3, known to affect hematopoiesis, and show that when immobilized on polypyrrole films, IL-3 is bioavailable for the bone marrow-derived FDC-P1 progenitor cell line. Moreover, IL-3 presentation can be successfully altered by changing the redox state of the film, in turn influencing FDC-P1 cell viability. This novel in vitro system provides a valuable tool for stimuli-responsive switchable protein presentation allowing the dissection of relevant mediators in stem and progenitor cell behavior.

Place, publisher, year, edition, pages
Cambridge: Royal Society of Chemistry, 2018. Vol. 6, p. 4665-4675
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Cell and Molecular Biology Cell Biology Biochemistry and Molecular Biology
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URN: urn:nbn:se:liu:diva-160752DOI: 10.1039/C8TB00782AOAI: oai:DiVA.org:liu-160752DiVA, id: diva2:1358068
Available from: 2019-10-07 Created: 2019-10-07 Last updated: 2019-10-07

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Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells(5006 kB)7 downloads
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Jönsson, Jan-IngvarJager, Edwin W. H.

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Jönsson, Jan-IngvarJager, Edwin W. H.
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Department of Physics, Chemistry and BiologyFaculty of Science & EngineeringFaculty of Medicine and Health SciencesDivision of Hematopoiesis and Developmental BiologySensor and Actuator Systems
Cell and Molecular BiologyCell BiologyBiochemistry and Molecular Biology

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3839404142434441 of 164
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