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Structural diffusion properties of two atypical Dps from the cyanobacterium Nostoc punctiforme disclose interactions with ferredoxins and DNA
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering. Uppsala Univ, Sweden.
Aix Marseille Univ, France; Inst Pasteur, France.
Uppsala Univ, Sweden.
Uppsala Univ, Sweden; Medicago AB, Sweden.
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2019 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1860, no 9, article id 148063Article in journal (Refereed) Published
Abstract [en]

Ferritin-like proteins, Dps (DNA-binding protein from starved cells), store iron and play a key role in the iron homeostasis in bacteria, yet their iron releasing machinery remains largely unexplored. The electron donor proteins that may interact with Dps and promote the mobilization of the stored iron have hitherto not been identified. Here, we investigate the binding capacity of the two atypical Dps proteins NpDps4 and NpDps5 from Nostoc punctiforme to isolated ferredoxins. We report NpDps-ferredoxin interactions by fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET) methods. Dynamic light scattering, size exclusion chromatography and native gel electrophoresis results show that NpDps4 forms a dodecamer at both pH 6M and pH 8.0, while NpDps5 forms a dodecamer only at pH 6.0. In addition, FCS data clearly reveal that the non-canonical NpDps5 interacts with DNA at pH 6.0. Our spectroscopic analysis shows that [Fe-S] centers of the three recombinantly expressed and isolated ferredoxins are properly incorporated and are consistent with their respective native states. The results support our hypothesis that ferredoxins could be involved in cellular iron homeostasis by interacting with Dps and assisting the release of stored iron.

Place, publisher, year, edition, pages
ELSEVIER , 2019. Vol. 1860, no 9, article id 148063
Keywords [en]
Cyanobacteria; Ferredoxin; Ferritin-like proteins; Fluorescence correlation spectroscopy, FCS; Iron; Protein-protein interaction
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-161002DOI: 10.1016/j.bbabio.2019.148063ISI: 000486134900006PubMedID: 31419396OAI: oai:DiVA.org:liu-161002DiVA, id: diva2:1367000
Note

Funding Agencies|NordForsk NCoE program "NordAqua" [82845]; Swedish Energy AgencySwedish Energy Agency [11674-5]; Carl Tryggers Foundation; Sven and Lilly Lawski foundation; European Research Council (ERC) under the European Unions Seventh Framework Programme (FP7/2007-2013)/ERC Grant [278242]; European Unions Horizon 2020 research and innovation programme [723241]

Available from: 2019-10-31 Created: 2019-10-31 Last updated: 2019-10-31

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