Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm-Ovis-Halomax during in vitro capacitation of cryopreserved ram spermatozoaVisa övriga samt affilieringar
2019 (Engelska)Ingår i: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 54, s. 46-49Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
This study aimed to compare the ability of sperm chromatin structure assay (SCSA (R)) and Sperm-Ovis-Halomax (R) to detect DNA fragmentation in frozen-thawed ram spermatozoa incubated under capacitating conditions in synthetic oviductal fluid (SOF) supplemented with oestrous sheep serum (SOF-ESS) at multiple time points (0-240 min). Incubation in SOF-ESS had no significant effects on SCSA (R) parameters while the percentage of spermatozoa with fragmented DNA measured by Sperm-Ovis-Halomax (R) increased after 180 min of incubation. In addition, no correlation or agreement was found between the techniques, suggesting that SCSA (R) and Sperm-Ovis-Halomax (R) may quantify different types of DNA damage in ram spermatozoa under these experimental conditions.
Ort, förlag, år, upplaga, sidor
WILEY , 2019. Vol. 54, s. 46-49
Nyckelord [en]
DNA integrity; sperm capacitation; SCSA (R); Sperm-Ovis-Halomax (R)
Nationell ämneskategori
Biofysik
Identifikatorer
URN: urn:nbn:se:liu:diva-161618DOI: 10.1111/rda.13519ISI: 000491448500007PubMedID: 31625230OAI: oai:DiVA.org:liu-161618DiVA, id: diva2:1367907
Anmärkning
Funding Agencies|ALF-Research, Region Ostergotland [LIO-698951]; Lions Forskningsfond [DNR LIU-2016-00641]; Linkoping and the Swedish Research Council FormasSwedish Research Council Formas [2017-00946]; Forskningsradet i Sydostra Sverige [473121, 745971]; Universidad de Castilla-La Mancha [2015/4062]
2019-11-052019-11-052021-12-28