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Chicken seminal fluid lacks CD9-and CD44-bearing extracellular vesicles
Linköping University, Department of Biomedical and Clinical Sciences, Division of Children's and Women's Health. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0003-2329-2635
Chattogram Vet and Anim Sci Univ, Bangladesh.
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2020 (English)In: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531Article in journal (Refereed) Epub ahead of print
Abstract [en]

The avian seminal fluid (SF) is a protein-rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm-free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non-enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9- nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2020.
Keywords [en]
CD44; CD9; electron microscopy; exosome precipitation; rooster; seminal fluid; tetraspanins; western blot
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-163630DOI: 10.1111/rda.13617ISI: 000508279900001PubMedID: 31894881OAI: oai:DiVA.org:liu-163630DiVA, id: diva2:1393837
Note

Funding Agencies|VetenskapsradetSwedish Research Council [2015-05919]; Forskningsradet i Sydostra Sverige [473121, 745971]; Lions Forskningsfond [DNR LIU-2016-00641]; Swedish Research Council FORMASSwedish Research CouncilSwedish Research Council Formas [2017-00946]

Available from: 2020-02-17 Created: 2020-02-17 Last updated: 2020-02-25Bibliographically approved

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Ntzouni, Maria

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Alvarez-Rodriguez, ManuelNtzouni, MariaWright, DominicMartinez-Serrano, CristinaRodriguez-Martinez, Heriberto
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Reproduction in domestic animals
Biophysics

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