Chicken seminal fluid lacks CD9-and CD44-bearing extracellular vesiclesVisa övriga samt affilieringar
2020 (Engelska)Ingår i: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 55, nr 3, s. 293-300Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
The avian seminal fluid (SF) is a protein-rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm-free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non-enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9- nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.
Ort, förlag, år, upplaga, sidor
Wiley-VCH Verlagsgesellschaft, 2020. Vol. 55, nr 3, s. 293-300
Nyckelord [en]
CD44; CD9; electron microscopy; exosome precipitation; rooster; seminal fluid; tetraspanins; western blot
Nationell ämneskategori
Biofysik
Identifikatorer
URN: urn:nbn:se:liu:diva-163630DOI: 10.1111/rda.13617ISI: 000508279900001PubMedID: 31894881OAI: oai:DiVA.org:liu-163630DiVA, id: diva2:1393837
Anmärkning
Funding Agencies|VetenskapsradetSwedish Research Council [2015-05919]; Forskningsradet i Sydostra Sverige [473121, 745971]; Lions Forskningsfond [DNR LIU-2016-00641]; Swedish Research Council FORMASSwedish Research CouncilSwedish Research Council Formas [2017-00946]
2020-02-172020-02-172023-12-28Bibliografiskt granskad