liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Synchronized tissue-scale vasculogenesis and ubiquitous lateral sprouting underlie the unique architecture of the choriocapillaris
Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Sun Yat Sen Univ, Peoples R China.
Fudan Univ, Peoples R China.
UCL Inst Ophthalmol, England.
Show others and affiliations
2020 (English)In: Developmental Biology, ISSN 0012-1606, E-ISSN 1095-564X, Vol. 457, no 2, p. 206-214Article in journal (Refereed) Published
Abstract [en]

The choriocapillaris is an exceptionally high density, two-dimensional, sheet-like capillary network, characterized by the highest exchange rate of nutrients for waste products per area in the organism. These unique morphological and physiological features are critical for supporting the extreme metabolic requirements of the outer retina needed for vision. The developmental mechanisms and processes responsible for generating this unique vascular network remain, however, poorly understood. Here we take advantage of the zebrafish as a model organism for gaining novel insights into the cellular dynamics and molecular signaling mechanisms involved in the development of the choriocapillaris. We show for the first time that zebrafish have a choriocapillaris highly similar to that in mammals, and that it is initially formed by a novel process of synchronized vasculogenesis occurring simultaneously across the entire outer retina. This initial vascular network expands by un-inhibited sprouting angiogenesis whereby all endothelial cells adopt tip-cell characteristics, a process which is sustained throughout embryonic and early post-natal development, even after the choriocapillaris becomes perfused. Ubiquitous sprouting was maintained by continuous VEGF-VEGFR2 signaling in endothelial cells delaying maturation until immediately before stages where vision becomes important for survival, leading to the unparalleled high density and lobular structure of this vasculature. Sprouting was throughout development limited to two dimensions by Bruchs membrane and the sclera at the anterior and posterior surfaces respectively. These novel cellular and molecular mechanisms underlying choriocapillaris development were recapitulated in mice. In conclusion, our findings reveal novel mechanisms underlying the development of the choriocapillaris during zebrafish and mouse development. These results may explain the uniquely high density and sheet-like organization of this vasculature.

Place, publisher, year, edition, pages
ACADEMIC PRESS INC ELSEVIER SCIENCE , 2020. Vol. 457, no 2, p. 206-214
Keywords [en]
Choriocapillaris; Vasculogenesis; Angiogenesis; Zebrafish; Development
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-164049DOI: 10.1016/j.ydbio.2019.02.002ISI: 000513075300007PubMedID: 30796893OAI: oai:DiVA.org:liu-164049DiVA, id: diva2:1411717
Note

Funding Agencies|Svenska Sallskapet for Medicinsk Forskning; Linkoping Universitet; Eva och Oscar Ahrens Stiftelse; Ollie och Elof Ericssons Stiftelse; Stiftelsen Sigurd och Elsa Goljes Minne; Magnus Bergvalls Stiftelse; Ogonfonden; Jeanssons Stiftelser; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81773059]; Shanghai Pujiang ProgramShanghai Pujiang Program [18PJ1400600]; VetenskapsradetSwedish Research Council

Available from: 2020-03-04 Created: 2020-03-04 Last updated: 2020-03-25

Open Access in DiVA

fulltext(3582 kB)20 downloads
File information
File name FULLTEXT01.pdfFile size 3582 kBChecksum SHA-512
be6fc8156b4202f2940e95bd51e29baffbe98a04efcd2a6945e4622d0be7186337b00961507af53fd4fa419d7e222ae041dda599609a9098338b2c506d5be639
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ali, ZaheerVazquez Rodriguez, GabrielaJensen, Lasse
By organisation
Division of Cardiovascular MedicineFaculty of Medicine and Health SciencesDivision of Surgery, Orthopedics and OncologyDivision of Diagnostics and Specialist MedicineDepartment of Clinical Pharmacology
In the same journal
Developmental Biology
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 20 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 53 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf