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Development of a reliable bioanalytical method based on prostate specific antigen trapping on the cavity of molecular imprinted polymer towards sensing of PSA using binding affinity of PSA-MIP receptor: A novel biosensor
Tabriz University of Medical Sciences, Iran.
Tabriz University of Medical Sciences, Iran.
Tabriz University of Medical Sciences, Iran.
Linköping University, Department of Science and Technology, Physics, Electronics and Mathematics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1829-8138
2020 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 188, article id 113447Article in journal (Refereed) Published
Abstract [en]

In this study, electrically-conducting poly [Toluidine Blue (PTB)] was applied as artificial receptor. It was organized by molecular imprinting approaches and via electrochemical technique for the sensitive monitoring of prostate-specific antigen (PSA). The protein-imprinted PTB was electropolymerized in a pre-formed glutaraldehyde-cysteamine (GA-Cys A) matrix on the surface of gold electrode, which significantly boosted the stability against degradation of the Molecular Imprinted Polymer (MIP) on the surface of pre-modified gold electrode. Moreover, the MIP bio-receptor ability towards protein recognition was explored by some electrochemical techniques. The binding affinity of MIP system was considerably upper than that of non-imprinted polymer (NIP) system, indicating the success of the method in generating imprinted materials that was specifically use to PSA protein. The incubation of the MIP modified electrode in various concentration of PSA (from 1-60 μg/L) resulted in the increase of the Fe (CN)63-/4- redox peak current. The bio-device also showed linear response from 1-60 μg/L and LLOQ of 1 μg/L by using DPV technique, leading to PSA monitoring in clinical samples. The proposed MIP-based biosensor was satisfactorily applied to the determination of PSA in human plasma samples. Therefore, the developed bio-device provides a new approach for sensitive, simple, rapid, and cost-effective monitoring of 1 μg/L of PSA. Notably, this approach could appear as an appropriate candidate for point-of-care (POC) use in clinical and biomedical analyses.

Place, publisher, year, edition, pages
Elsevier, 2020. Vol. 188, article id 113447
Keywords [en]
Binding affinity, Biomacromolecules, Biomedical analysis, Biosensor, Encapsulation, Prostate cancer.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-168893DOI: 10.1016/j.jpba.2020.113447ISI: 000556785200040PubMedID: 32623317OAI: oai:DiVA.org:liu-168893DiVA, id: diva2:1463560
Note

Funding agencies:  Tabriz University of Medical Sciences

Available from: 2020-09-02 Created: 2020-09-02 Last updated: 2023-11-17
In thesis
1. (Nano)materials for electrochemical sensing applications in different fields
Open this publication in new window or tab >>(Nano)materials for electrochemical sensing applications in different fields
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Health care and the medical industry, environmental monitoring and food sector are just a few examples of human’s major concerns. Design and analytical applications of (bio)sensors and sensing (nano)materials in these fields which have drawn tremendous attention by scientific community, require multidisciplinary knowledge in materials, transducers, and measurement methodologies. Among different types of transducers, electrochemical transducers have gained a lot of interest due to their simplicity, ease of fabrication and integration, selectivity, and sensitivity. Application of sensing materials, in particular nanomaterials, provide several improvements for analytical sensing such as selectivity, sensitivity, robustness, and fast response. Therefore, different nanomaterials due to their excellent characterization are exploited as key instruments for the design of high-performant analytical assays in various fields.

Considering this established nanomaterial-based electrochemical sensing applications as alternatives to conventional methods in various fields, in this thesis, we synthesized and characterized some (nano)materials such as gold nanostars (AuNSs), iron oxide/zinc oxide (α-Fe2O3/ZnO) nanocomposite for sensing applications in environmental monitoring. We fabricated screen printed Ruthenium (IV) oxide (RuO2-Nf) pH electrodes and applied them for continuous pH monitoring in milk and during milk coagulation. Although the results indicated the applicability of the RuO2-Nf electrodes for continuous pH measurement in milk as a replacement of traditional glass electrode, they are not suitable for more viscous samples. Later we investigated the cleaning procedure for these pH electrodes, and we showed that soaking them in the solution of 5% pepsin in 0.1 M HCl restores the electrodes to their initial state for pH measurement in food samples. In another work we developed a molecular imprinted polymer-based biosensor for successful detection of prostate-specific antigen. At the end we investigated the effect of pH on the growth of pathogenic bacteria using electrical impedance spectroscopy.

Abstract [sv]

Hälso- och sjukvårdsbranschen, miljöövervakning och livsmedelssektorn är bara några exempel på mänsklighetens stora bekymmer. Design och analytiska tillämpningar av (bio)sensorer och sensormaterial inom dessa områden, som har lockat enorm uppmärksamhet från vetenskapssamhället, kräver tvärvetenskaplig kunskap inom material, omvandlare och mätmetodik. Bland olika typer av omvandlare har elektrokemiska (bio)sensorer väckt stort intresse på grund av sin enkelhet, lätthet att tillverka, selektivitet och känslighet. Användning av nya sensormaterial, särskilt nanomaterial, ger flera förbättringar för analytisk detektion, som exempelvis selektivitet, känslighet, robusthet och snabb respons. Därför utnyttjas olika nanomaterial på grund av deras utmärkta egenskaper som nyckelkomponenter för designen av högpresterande analytiska metoder inom olika områden.

Med tanke på att nanomaterialbaserade elektrokemiska sensorapplikationer är ett hett ämne har vi i denna avhandling syntetiserat och karaktäriserat vissa nanomaterial såsom guld nanostjärnor (AuNSs) och järnoxid/zinkoxid (α-Fe2O3/ZnO) nanokomposit för sensormaterial i miljöövervakning. Vi tillverkade tryckta pH-elektroder av rutenium (IV)oxid (RuO2-Nf) och använde dem för kontinuerlig pH-övervakning i mjölk, och under mjölkkoagulering. Även om resultaten indikerade att RuO2-Nf-elektroderna är användbara för kontinuerlig pH-mätning i mjölk som en ersättning för traditionella glaselektroder, är de inte lämpliga för mer viskösa prov. Senare undersökte vi rengöringsmetoden för dessa pH-elektroder och visade att blötläggning i en lösning av 5% pepsin i 0,1 M HCI återställer elektroderna till sitt ursprungliga tillstånd för pH-mätning i livsmedelsprover. I ett annat arbete utvecklade vi en molekylär imprintingpolymerbaserad biosensor för framgångsrik detektion av prostata-specifikt antigen. Till slut undersökte vi effekten av pH på tillväxten av patogena bakterier med hjälp av elektrisk impedansspektroskopi.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2023. p. 47
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 2336
Keywords
Electrochemical (bio)sensors, Nanomaterials, AuNSs, (α-Fe2O3/ZnO) nanocomposite, (RuO2-Nf) pH electrodes, MIP technology
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-199206 (URN)10.3384/9789180752985 (DOI)9789180752978 (ISBN)9789180752985 (ISBN)
Public defence
2023-10-25, K1, Kåkenhus, Campus Norrköping, Norrköping, 09:30 (English)
Opponent
Supervisors
Note

Funding agency: The European Commission through the Marie Skłodowska-Curie Actions program

Available from: 2023-11-17 Created: 2023-11-17 Last updated: 2024-05-08Bibliographically approved

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