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The FORCE Panel: An All-in-One SNP Marker Set for Confirming Investigative Genetic Genealogy Leads and for General Forensic Applications
Linköping University, Department of Biomedical and Clinical Sciences, Division of Molecular Medicine and Virology. Linköping University, Faculty of Medicine and Health Sciences. Natl Board Forens Med, Dept Forens Genet & Forens Toxicol, SE-58758 Linkoping, Sweden.
Armed Forces Med Examiner Syst Armed Forces DNA I, DE 19902 USA; SNA Int LLC, VA 22314 USA; Uppsala Univ, Sweden.
Armed Forces Med Examiner Syst Armed Forces DNA I, DE 19902 USA; SNA Int LLC, VA 22314 USA.
Armed Forces Med Examiner Syst Armed Forces DNA I, DE 19902 USA; SNA Int LLC, VA 22314 USA.
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2021 (English)In: Genes, ISSN 2073-4425, E-ISSN 2073-4425, Vol. 12, no 12, article id 1968Article in journal (Refereed) Published
Abstract [en]

The FORensic Capture Enrichment (FORCE) panel is an all-in-one SNP panel for forensic applications. This panel of 5422 markers encompasses common, forensically relevant SNPs (identity, ancestry, phenotype, X- and Y-chromosomal SNPs), a novel set of 3931 autosomal SNPs for extended kinship analysis, and no clinically relevant/disease markers. The FORCE panel was developed as a custom hybridization capture assay utilizing ~20,000 baits to target the selected SNPs. Five non-probative, previously identified World War II (WWII) cases were used to assess the kinship panel. Each case included one bone sample and associated family reference DNA samples. Additionally, seven reference quality samples, two 200-year-old bone samples, and four control DNAs were processed for kit performance and concordance assessments. SNP recovery after capture resulted in a mean of ~99% SNPs exceeding 10X coverage for reference and control samples, and 44.4% SNPs for bone samples. The WWII case results showed that the FORCE panel could predict first to fifth degree relationships with strong statistical support (likelihood ratios over 10,000 and posterior probabilities over 99.99%). To conclude, SNPs will be important for further advances in forensic DNA analysis. The FORCE panel shows promising results and demonstrates the utility of a 5000 SNP panel for forensic applications.

Place, publisher, year, edition, pages
MDPI , 2021. Vol. 12, no 12, article id 1968
Keywords [en]
SNP; kinship; massively parallel sequencing; next generation sequencing; hybridization capture; bone
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:liu:diva-182388DOI: 10.3390/genes12121968ISI: 000738991400001PubMedID: 34946917OAI: oai:DiVA.org:liu-182388DiVA, id: diva2:1630323
Available from: 2022-01-20 Created: 2022-01-20 Last updated: 2022-02-09

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Tillmar, Andreas
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