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Detection of global glycosylation changes of serum proteins in type 1 diabetes using a lectin panel and multivariate data analysis
Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Pediatrik. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Pediatrik. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Barn- och kvinnocentrum, Barn- och ungdomskliniken i Linköping.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
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2008 (Engelska)Ingår i: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 76, nr 2, s. 333-337Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Global glycosylation changes of serum proteins in type 1 diabetic patients have in this paper been investigated based on the interaction of the saccharide moiety of serum proteins with different lectins. Lectins are proteins, which bind carbohydrates specifically and reversibly. Panels with lectins of various carbohydrate specificities were immobilized on gold surfaces. Sera from healthy individuals, newly diagnosed type 1 diabetes patients and type 1 diabetes patients having had the disease for 4–6 years, respectively, were applied to the lectin panel. The biorecognition was evaluated with null ellipsometry. Data obtained were related to an internal standard of lactoferrin. Multivariate data analysis (MVDA) techniques were used to analyze data.

Principal component analysis showed that the lectin panel enabled discrimination between sera from the three different above-mentioned groups. Using an artificial neuronal net (ANN), it was possible to correctly categorize unknown serum samples into one of the three groups.

 

Ort, förlag, år, upplaga, sidor
2008. Vol. 76, nr 2, s. 333-337
Nyckelord [en]
Lectin panel, Glycosylation changes, Type 1 diabetes, Ellipsometry, MVDA
Nationell ämneskategori
Kemi
Identifikatorer
URN: urn:nbn:se:liu:diva-12468DOI: 10.1016/j.talanta.2008.02.046OAI: oai:DiVA.org:liu-12468DiVA, id: diva2:180
Tillgänglig från: 2008-09-06 Skapad: 2008-09-06 Senast uppdaterad: 2017-12-13Bibliografiskt granskad
Ingår i avhandling
1. Interaction Studies in Complex Fluids with Optical Biosensors
Öppna denna publikation i ny flik eller fönster >>Interaction Studies in Complex Fluids with Optical Biosensors
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

In this thesis interactions in complex fluids, such as serum and meat juice, were analysed with optical biosensor techniques.

Panels of lectins immobilised on gold surfaces were used for investigation of differences in protein glycosylation pattern in sera and meat juices between various species. The present panel was also used for investigation of global glycosylation changes of serum proteins in type 1 diabetes patients. Biorecognition was evaluated with null ellipsometry and scanning ellipsometry combined with multivariate data analysis techniques (MVDA). Principal component analysis (PCA) showed that the lectin panel enabled discrimination between sera from the different species as well as for the different meat juices. The results also indicate that there is a measurable global alteration in glycosylation pattern of serum proteins in type 1 diabetic patients compared to healthy subjects. Using an artificial neuronal net (ANN), it was also possible to correctly categorise unknown serum samples into their respective class or group. The analytical potential of combining information from lectin panels with multivariate data analysis was thereby demonstrated.

Also, a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D) has been developed. When measuring trace molecules, such as autoantibodies, in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labelled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (from 4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA. Finally, the assay was used in a screening study of newly diagnosed type 1 diabetes patients and non-diabetic subjects.

Ort, förlag, år, upplaga, sidor
Linköping: Linköping University Electronic Press, 2008. s. 70
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1195
Nyckelord
Type 1 diabetes, Insulin, Lectin panel, Ellipsometry, Meat juice, Serum proteins
Nationell ämneskategori
Kemi
Identifikatorer
urn:nbn:se:liu:diva-14694 (URN)978-91-7394-852-5 (ISBN)
Disputation
2008-09-19, Planck, Fysikhuset, Campus Norrköping, Linköpings universitet, Linköping, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2008-09-26 Skapad: 2008-09-19 Senast uppdaterad: 2009-04-24Bibliografiskt granskad

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Carlsson, JennyGullstrand, CamillaLudvigsson, JohnnyLundström, IngemarWinquist, Fredrik

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Carlsson, JennyGullstrand, CamillaLudvigsson, JohnnyLundström, IngemarWinquist, Fredrik
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Tillämpad FysikTekniska högskolanPediatrikHälsouniversitetetBarn- och ungdomskliniken i Linköping
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Talanta: The International Journal of Pure and Applied Analytical Chemistry
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