This study is aimed at investigating those parameters related to timber fooring that can afect the acceptability of vibration behaviour of a timber foor in a residential building in view of the criteria stated in Eurocode EC5. The timber foor investigated is made of OSB/3 foorboards and timber joists. The parameters that are investigated in this study are thickness of fooring, foor joist span, joist spacing and connection of foorboards to the joists. In this context, two cases are considered. First: the fooring is nailed or screwed to the joists and no composite action or interaction is obtained between joists and foorboards. Second: the fooring is glued sufciently to the joists and full interaction is obtained. The result suggests that glued foorboards perform much better with respect to natural frequency, static defection and peak foor velocity than nailed or screwed foorboards. In almost all cases of glued foorboards, the result complies fully with the Eurocode 5 design vibration requirements. However, as foor lengths increase, the static defection will increase beyond the allowable limit, especially for relatively thin foor panels and relatively widely spread joists. For both cases, increasing foorboards thickness and decreasing the joist span by adding more beams can yield even better results to satisfy the requirement of vibration comfort.