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A cell-based sensor system for toxicity testing using multiwavelength fluorescence spectroscopy
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-9711-794X
2009 (engelsk)Inngår i: ANALYTICAL BIOCHEMISTRY, ISSN 0003-2697, Vol. 387, nr 2, s. 271-275Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A novel cell-based fluorometric sensor system for toxicity monitoring is described, which uses functional spontaneously contracting cardiomyocytes (HL-1 cell line) as the biological recognition element. Based on these highly specialized cells, it has the potential of providing a sensitive and relevant analytical in vitro toxicity testing method. The system was configured by propagating the surface-attaching HL-1 cardiomyocytes in the wells of a 96-well microtiter plate and connecting the plate via an optical fiber to a fluorescence spectrometer capable of excitation-emission matrix scanning. The fluorescence data were analyzed using a conventional spectral analysis software program. The performance of the system for detection of general cytotoxicity to the cells was evaluated using three well-known drugs: verapamil, quinidine, and acetaminophen. The dose-response curves were assessed and the EC50 values were determined (0.10 +/- 0.007, 0.23 +/- 0.025, and 12.32 +/- 2.40 mM, respectively). Comparison with in vitro and in vivo reference data for the drugs showed good correlations, suggesting that this cell-based sensor system Could be a useful tool in pharmacological in vitro drug testing.

sted, utgiver, år, opplag, sider
2009. Vol. 387, nr 2, s. 271-275
Emneord [en]
Autofluorescence, HL-1 cell line, Cardiomyocytes, Cytotoxicity screening, Drug testing, Microtiter plate, Tryptophan fluorescence, In vitro toxicity
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-17736DOI: 10.1016/j.ab.2009.01.027OAI: oai:DiVA.org:liu-17736DiVA, id: diva2:211739
Tilgjengelig fra: 2009-04-17 Laget: 2009-04-17 Sist oppdatert: 2019-01-22

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