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Decoration of amyloid fibrils with luminescent conjugated polymers
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
University of Lund.
University of Lund.
University of Lund.
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2008 (Engelska)Ingår i: Journal of Materials Chemistry, ISSN 0959-9428, E-ISSN 1364-5501, Vol. 18, nr 1, s. 126-132Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In this work we report the coating of a biological template with a polar, but uncharged, luminescent conjugated polymer, soluble in organic solvents but not in water, to produce a nanowire. Amyloid fibrils from bovine insulin were decorated with an alternating polyfluorene derivative. Decorated fibrils were partially aligned on hydrophobic surfaces as separate and bundled fibrils, by means of molecular combing. The single molecule spectroscopy technique utilizing excitation by rotating linearly polarized light and fluorescence detection through a rotating polarizer showed a high degree of anisotropy of the polymer chains on the individual fibrils. The high degree of polarization indicated highly oriented polymer chains with the preferential orientation of the polymer backbone along the fibrils. The anisotropy ratios are comparable with those of well-oriented polymer chains in films. © The Royal Society of Chemistry.

Ort, förlag, år, upplaga, sidor
2008. Vol. 18, nr 1, s. 126-132
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-14603DOI: 10.1039/b712829kISI: 000251580400016OAI: oai:DiVA.org:liu-14603DiVA, id: diva2:23996
Tillgänglig från: 2007-10-12 Skapad: 2007-10-12 Senast uppdaterad: 2017-12-13
Ingår i avhandling
1. Conjugated Polymers, Amyloid Detection and Assembly of Biomolecular Nanowires
Öppna denna publikation i ny flik eller fönster >>Conjugated Polymers, Amyloid Detection and Assembly of Biomolecular Nanowires
2007 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The research field of conjugated polymers has grown due to the optical and electronic properties of the material, useful in applications such as solar cells and printed electronics, but also in biosensors and for interactions with biomolecules. In this thesis conjugated polymers have been used in two related topics; to detect conformational changes in proteins and to assemble the polymers with biomolecules into nanowires.

Within biosensing, conjugated polymers have been used for detection of a wide range of biological events, such as DNA hybridization or enzymatic activity, utilizing both electronic and optical changes in the polymer. Here the focus has been to use the polymers as optical probes to discriminate between native and misfolded protein, as well as to follow the misfolding processes in vitro. The understanding and detection of protein misfolding, for example amyloid fibril formation, is a topic of growing importance. The misfolding process is strongly associated with several devastating diseases such as Alzheimer’s disease, Parkinson’s disease and Bovine Spongiform Encephalopathy (BSE). We have developed detection schemes for discrimination between proteins in the native or amyloid fibril state based on luminescent polythiophene derivatives. Through a synthesis strategy based on polymerization of trimer blocks rather than of monomers, polythiophene derivatives with higher optical signal specificity for amyloid-like fibrils were obtained.

Self-assembly of nanowires containing conjugated polymers is a route to generate structures of unique opto-electrical characteristics without the need for tedious topdown processes. Biomolecules can have nanowire geometries of extraordinary aspect ratio and functionalities. The DNA molecule is the most well known and exploited of these. In this thesis work the more stable amyloid fibril has been used as a template to organize conjugated polymers. Luminescent, semi-conducting, conjugated polymers have been incorporated in and assembled onto amyloid fibrils. Using luminescence quenching we have demonstrated that the conjugated material can retain the electro-activity after the incorporation process. Furthermore, the amyloid fibril/conjugated polymer hybrid structures can be organized on surfaces by the means of molecular combing and soft lithography.

In the process of generating self-assembled biomolecular nanowires functionalized with conjugated polymers, we have shown a new synthesis strategy for a water-soluble highly conducting polythiophene derivative. This material, PEDOT-S, has shown affinity for amyloid fibrils, but can also be very useful in conventional opto-electronic polymer-based devices.

Ort, förlag, år, upplaga, sidor
Institutionen för fysik, kemi och biologi, 2007
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1117
Nyckelord
Conjugated Polymers, Amyloid Fibrils, Nanowires, Self-Assembly, Sensor, Amyloid Detection
Nationell ämneskategori
Fysik
Identifikatorer
urn:nbn:se:liu:diva-9577 (URN)978-91-85831-42-5 (ISBN)
Disputation
2007-09-04, Planck, Physics Building, Linköping University, Linköping, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2007-10-12 Skapad: 2007-10-12 Senast uppdaterad: 2009-04-29

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Herland, AnnaInganäs, Olle

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