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Interactions between the juxtamembrane domain of the EGFR and calmodulin measured by surface plasmon resonance
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för medicin och vård, Farmakologi.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
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2002 (engelsk)Inngår i: Cellular Signalling, ISSN 0898-6568, E-ISSN 1873-3913, Vol. 14, nr 12, s. 1005-1013Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

One early response to epidermal growth factor receptor (EGFR) activation is an increase in intracellular calcium. We have used surface plasmon resonance (SPR) to study real-time interactions between the intracellular juxtamembrane (JM) region of EGFR and calmodulin. The EGFR-JM (Met644-Phe688) was expressed as a GST fusion protein and immobilised on a sensor chip surface. Calmodulin specifically interacts with EGFR-JM in a calcium-dependent manner with a high on and high off rate. Chemical modification of EGFR-JM by using arginine-selective phenylglyoxal or deletion of the basic segment Arg645-Arg657 inhibits the interaction. Phosphorylation of EGFR-JM by protein kinase C (PKC) or glutamate substitution of Thr654 inhibits the interaction, suggesting that PKC phosphorylation electrostatically interferes with calmodulin binding to basic arginine residues. Calmodulin binding was also inhibited by suramin. Our results suggest that EGFR-JM is essential for epidermal growth factor (EGF)-mediated calcium-calmodulin signalling and for signal integration between other signalling pathways.

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2002. Vol. 14, nr 12, s. 1005-1013
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URN: urn:nbn:se:liu:diva-27056DOI: 10.1016/S0898-6568(02)00034-7Lokal ID: 11702OAI: oai:DiVA.org:liu-27056DiVA, id: diva2:247607
Tilgjengelig fra: 2009-10-08 Laget: 2009-10-08 Sist oppdatert: 2017-12-13

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Nilsson, Ulrica KLiedberg, BoSvensson, Samuel

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