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Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Experimentell patologi. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Ortopedi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Centrum för kirurgi, ortopedi och cancervård, Ortopedkliniken i Linköping.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Ortopedi. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Ortopedi. Linköpings universitet, Hälsouniversitetet.
2002 (engelsk)Inngår i: Wound Repair and Regeneration, ISSN 1067-1927, E-ISSN 1524-475X, Vol. 10, nr 5, s. 336-340Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The influence of platelet-rich plasma lysates on fibroblast proliferation was studied in culture. Cells were exposed to platelet lysates that had been preincubated at different pHs (5.0, 7.1, and 7.6). Proliferation was evaluated with the MTT assay and incorporation of [3H]thymidine into macromolecules, while type I collagen production was assayed by Western blotting. Enzyme-linked immunosorbent assays were used to determine platelet-derived growth factor and transforming growth factor-β concentrations. Platelets preincubated in an acidic environment (pH 5.0) induced the highest degree of fibroblast proliferation, and the concentration of platelet-derived growth factor in the different treated lysates was the highest at that particular pH. The concentration of transforming growth factor-β, however, was lower after incubation at pH 5.0 than at either pH 7.1 or 7.6. These findings may be relevant to normal wound healing in vivo and useful in the treatment of wounds and delayed healing processes.

sted, utgiver, år, opplag, sider
2002. Vol. 10, nr 5, s. 336-340
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-27830DOI: 10.1046/j.1524-475X.2002.10510.xISI: 000178921600010Lokal ID: 12587OAI: oai:DiVA.org:liu-27830DiVA, id: diva2:248382
Tilgjengelig fra: 2009-10-08 Laget: 2009-10-08 Sist oppdatert: 2017-12-13
Inngår i avhandling
1. Effects of pH on platelets release of growth factors in wound healing
Åpne denne publikasjonen i ny fane eller vindu >>Effects of pH on platelets release of growth factors in wound healing
2003 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Wound healing is thought to involve a complex series of interactions between biochemical mediators, cells and extracellular matrix. Known to stimulate cellular movement, proliferation and biosynthetic activity, growth factors may play a significant role in stimulating wound repair. This thesis focuses on the early phase of wound healing. We established an experimental model which mimics the early proliferative stage and concentrated on a potentially interesting aspect of the wound environment pH as a modulator of the release of growth factors from platelets. The influence of platelet-rich plasma lysates on fibroblast proliferation at varying pH was studied in culture. We found that the concentration of platelet-derived growth factor in the different lysates was highest at pH 5.0. The concentration of transforming growth factor beta, however, was lower after incubation at pH 5.0 than at pH 7.1 and 7.6. Fibroblast proliferation and collagen production were enhanced by platelet lysates at pH 5.0, 7.1 and 7.6.We also studied the release of growth factors from platelet supernatants which were thrombin-activated at pH 7.4, then the pH was lowered to 5.0, remained the same or was raised to 8.5, or were thrombin-activated (or not) at pH 5.0, 7.4 and 8.5. Our results suggest that release of growth factors is dependent not only on platelet activation, but also on long-term incubation at 37°C and change in pH. ln summary, we found that pH may influence the activity of platelet proteins, resulting in fibroblast growth and promotion of wound healing.

sted, utgiver, år, opplag, sider
Linköping: Linköpings universitet, 2003. s. 46
Serie
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 59
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-28096 (URN)12862 (Lokal ID)91-7373-590-2 (ISBN)12862 (Arkivnummer)12862 (OAI)
Tilgjengelig fra: 2009-10-08 Laget: 2009-10-08 Sist oppdatert: 2013-09-12

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