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Adsorption of human serum albumin in porous silicon gradients
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad optik.ORCID-id: 0000-0001-9229-2028
2003 (engelsk)Konferansepaper, Publicerat paper (Annet vitenskapelig)
Abstract [en]

Backside etching has been utilized to produce gradients of pore size and layer thickness in porous silicon. Human serum albumin (HSA) was adsorbed on such gradients at two different pH values: 4.9, the pI of HSA, and 7.4, the physiological pH. The samples were investigated by scanning electron microscopy, spectroscopic ellipsometry, and autoradiography. The results show that the protein adsorbed displays a gradient along with the pore size and the thickness gradient. The higher than current density used during etching, the more sway-back shaped curves were seen for the protein adsorption pattern, independent of pH. When 50 mA/cm2 current density was used during etching, the quota between the maximal intensity value and the plateau value seen after adsorption of the HSA increased for pH 7.4.

sted, utgiver, år, opplag, sider
2003. Vol. 197, nr 2, s. 326-330
HSV kategori
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URN: urn:nbn:se:liu:diva-46654DOI: 10.1002/pssa.200306518OAI: oai:DiVA.org:liu-46654DiVA, id: diva2:267550
Tilgjengelig fra: 2009-10-11 Laget: 2009-10-11 Sist oppdatert: 2014-01-28

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