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Gas sensor arrays for early detection of infection in mammalian cell culture
Cell Culture Production, Pharmacia AB, S-112 87 Stockholm, Sweden.
Cell Culture Production, Pharmacia AB, S-112 87 Stockholm, Sweden.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi.ORCID-id: 0000-0001-9711-794X
2002 (engelsk)Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 17, nr 5, s. 395-403Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The detection of bacterial infections in a mammalian cell culture process is realised using a gas sensor array. In production-scale and laboratory-scale cultivations of a perfused recombinant CHO-cell culture producing human blood coagulation Factor VIII, we show that the gas sensor array identifies bacterial contamination earlier than conventional methods. The sensitivity of the instrument is verified by inoculation of a blank cell culture medium with defined bacterial cell counts. © 2002 Elsevier Science B.V. All rights reserved.

sted, utgiver, år, opplag, sider
2002. Vol. 17, nr 5, s. 395-403
Emneord [en]
Bioprocess contamination, Gas sensor array, On-line monitoring
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-47059DOI: 10.1016/S0956-5663(01)00315-3OAI: oai:DiVA.org:liu-47059DiVA, id: diva2:267955
Tilgjengelig fra: 2009-10-11 Laget: 2009-10-11 Sist oppdatert: 2019-01-22

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