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Mass Spectrometric Resolution of Reversible Protein Phosphorylation in Photosynthetic Membranes of Arabidopsis thaliana
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi.
Univ. of Wisconsin Biotech. Center, University of Wisconsin, Madison, WI 53706, United States.
Cell. and Molecular Biology Program, Department of Horticulture, University of Wisconsin, Madison, WI 53706, United States, Univ. of Wisconsin Biotech. Center, University of Wisconsin, Madison, WI 53706, United States.
Cell. and Molecular Biology Program, Department of Horticulture, University of Wisconsin, Madison, WI 53706, United States, Dept. of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706, United States.
2001 (Engelska)Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 276, nr 10, s. 6959-6966Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The use of mass spectrometry to characterize the phosphorylome, i.e. the constituents of the proteome that become phosphorylated, was demonstrated using the reversible phosphorylation of chloroplast thylakoid proteins as an example. From the analysis of tryptic peptides released from the surface of Arabidopsis thylakoids, the principal phosphoproteins were identified by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. These studies revealed that the Dl, D2, and CP43 proteins of the photosystem II core are phosphorylated at their N-terminal threonines (Thr), the peripheral PsbH protein is phosphorylated at Thr-2, and the mature light-harvesting polypeptides LCHII are phosphorylated at Thr-3. In addition, a doubly phosphorylated form of PsbH modified at both Thr-2 and Thr-4 was detected. By comparing the levels of phospho- and nonphosphopeptides, the in vivo phosphorylation states of these proteins were analyzed under different physiological conditions. None of these thylakoid proteins were completely phosphorylated in the steady state conditions of continuous light or completely dephosphorylated after a long dark adaptation. However, rapid reversible hyperphosphorylation of PsbH at Thr-4 in response to growth in light/dark transitions and a pronounced specific dephosphorylation of the Dl, D2, and CP43 proteins during heat shock was detected. Collectively, our data indicate that changes in the phosphorylation of photosynthetic proteins are more rapid during heat stress than during normal light/ dark transitions. These mass spectrometry methods offer a new approach to assess the stoichiometry of in vivo protein phosphorylation in complex samples.

Ort, förlag, år, upplaga, sidor
2001. Vol. 276, nr 10, s. 6959-6966
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Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-47457DOI: 10.1074/jbc.M009394200OAI: oai:DiVA.org:liu-47457DiVA, id: diva2:268353
Tillgänglig från: 2009-10-11 Skapad: 2009-10-11 Senast uppdaterad: 2017-12-13

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