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Different Requirements for Early and Late Phases of Azurophilic Granule-Phagosome Fusion
Lund University.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
Lund University.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
Vise andre og tillknytning
2009 (engelsk)Inngår i: TRAFFIC, ISSN 1398-9219, Vol. 10, nr 12, s. 1881-1893Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts or calcium. These findings were found to be true also when using Streptococcus pyogenes bacteria as prey, and depletion of calcium affected the kinetics of bacterial intracellular survival. These findings suggest that the mechanisms for delivery of azurophilic content to nascent and sealed phagosomes, respectively, differ in their dependence on calcium and membrane rafts.

sted, utgiver, år, opplag, sider
2009. Vol. 10, nr 12, s. 1881-1893
Emneord [en]
azurophilic granules, calcium, HL-60 cells, membrane rafts, membrane traffic, neutrophil, phagosome
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-52369DOI: 10.1111/j.1600-0854.2009.00986.xOAI: oai:DiVA.org:liu-52369DiVA, id: diva2:282052
Tilgjengelig fra: 2009-12-18 Laget: 2009-12-18 Sist oppdatert: 2009-12-18

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Totalt: 31 treff
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